Bioproduction of trans-Aconitic Acid from Citric Acid by Whole-Cell Reaction of Escherichia coli Heterologously Expressing the Aconitate Isomerase Gene from Pseudomonas sp. WU-0701

Keiichi Kobayashi, Junya Maruebi, Kotaro Kirimura

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Aconitate isomerase (AI; EC 5.3.3.7) catalyzes the isomerization of cis-aconitic acid and trans-aconitic acid. Since trans-aconitic acid is an unsaturated organic acid, effective and environmentally benign processes are needed for trans-aconitic acid production. Here, the genes encoding AI from Pseudomonas sp. WU-0701 and aconitate hydratase (AH; EC 4.2.1.3) from Escherichia coli W3110, catalyzing the dehydration of citric acid and formation of cis-aconitic acid, were co-expressed in E. coli Rosetta 2(DE3). The recombinant E. coli cells were used as biocatalysts for trans-aconitic acid production from citric acid by whole-cell reaction. The optimal conditions were 37°C and pH 7.0. Using recombinant E. coli cells as biocatalysts for the whole-cell reaction, 91 mM trans-aconitic acid was produced from 400 mM citric acid within 120 min. This is the first report describing a solvent- and harmful reagent-free system for trans-aconitic acid bioproduction usable under moderate conditions.

    Original languageEnglish
    Pages (from-to)1467-1471
    Number of pages5
    JournalChemistrySelect
    Volume1
    Issue number7
    DOIs
    Publication statusPublished - 2016 Jan 1

    Fingerprint

    Aconitic Acid
    Isomerases
    Citric Acid
    Escherichia coli
    Genes
    Aconitate Hydratase
    Gene encoding
    Organic acids
    Enzymes
    Isomerization
    Dehydration

    Keywords

    • aconitate isomerase
    • biocatalyst
    • citric acid
    • Pseudomonas sp.
    • trans-aconitic acid

    ASJC Scopus subject areas

    • Chemistry(all)

    Cite this

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    abstract = "Aconitate isomerase (AI; EC 5.3.3.7) catalyzes the isomerization of cis-aconitic acid and trans-aconitic acid. Since trans-aconitic acid is an unsaturated organic acid, effective and environmentally benign processes are needed for trans-aconitic acid production. Here, the genes encoding AI from Pseudomonas sp. WU-0701 and aconitate hydratase (AH; EC 4.2.1.3) from Escherichia coli W3110, catalyzing the dehydration of citric acid and formation of cis-aconitic acid, were co-expressed in E. coli Rosetta 2(DE3). The recombinant E. coli cells were used as biocatalysts for trans-aconitic acid production from citric acid by whole-cell reaction. The optimal conditions were 37°C and pH 7.0. Using recombinant E. coli cells as biocatalysts for the whole-cell reaction, 91 mM trans-aconitic acid was produced from 400 mM citric acid within 120 min. This is the first report describing a solvent- and harmful reagent-free system for trans-aconitic acid bioproduction usable under moderate conditions.",
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    author = "Keiichi Kobayashi and Junya Maruebi and Kotaro Kirimura",
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    AU - Kobayashi, Keiichi

    AU - Maruebi, Junya

    AU - Kirimura, Kotaro

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    N2 - Aconitate isomerase (AI; EC 5.3.3.7) catalyzes the isomerization of cis-aconitic acid and trans-aconitic acid. Since trans-aconitic acid is an unsaturated organic acid, effective and environmentally benign processes are needed for trans-aconitic acid production. Here, the genes encoding AI from Pseudomonas sp. WU-0701 and aconitate hydratase (AH; EC 4.2.1.3) from Escherichia coli W3110, catalyzing the dehydration of citric acid and formation of cis-aconitic acid, were co-expressed in E. coli Rosetta 2(DE3). The recombinant E. coli cells were used as biocatalysts for trans-aconitic acid production from citric acid by whole-cell reaction. The optimal conditions were 37°C and pH 7.0. Using recombinant E. coli cells as biocatalysts for the whole-cell reaction, 91 mM trans-aconitic acid was produced from 400 mM citric acid within 120 min. This is the first report describing a solvent- and harmful reagent-free system for trans-aconitic acid bioproduction usable under moderate conditions.

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