Biosynthesis of Thermoresponsive Magnetic Nanoparticles by Magnetosome Display System

Tomoko Yoshino, Takumi Shimada, Yasuhito Ito, Toru Honda, Yoshiaki Maeda, Tadashi Matsunaga, Tsuyoshi Tanaka

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3 Citations (Scopus)

Abstract

Thermoresponsive magnetic nanoparticles (MNPs) were synthesized using a magnetosome display system. An elastin-like polypeptide decamer of VPGVG (ELP10), which is hydrophobic above the transition temperature (Tt) and can form an insoluble aggregation, was immobilized on biogenic MNPs in the magnetotactic bacterium, Magnetospirillum magneticum AMB-1. It was suggested that hydrophobicity of the MNP surface increased at 60 °C compared with 20 °C by the immobilization of ELP10. Size distribution analysis indicated that the immobilization of ELP10 onto MNPs induced the increased hydrophobicity with increasing temperatures up to 60 °C, promoting aggregation of the particles by hydrophobic and magnetic interactions. These results suggest that the acceleration of magnetic collection at 60 °C was caused by particle aggregation promoted by hydrophobic interaction between ELP-MNPs. Furthermore, the immobilization of ELP on MNPs gave a quick magnetic collection at 60 °C by external magnetic field. The thermoresponsive properties will further expand the utility of biotechnological applications of biogenic MNPs.

Original languageEnglish
Pages (from-to)1756-1762
Number of pages7
JournalBioconjugate Chemistry
Volume29
Issue number5
DOIs
Publication statusPublished - 2018 May 16
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Yoshino, T., Shimada, T., Ito, Y., Honda, T., Maeda, Y., Matsunaga, T., & Tanaka, T. (2018). Biosynthesis of Thermoresponsive Magnetic Nanoparticles by Magnetosome Display System. Bioconjugate Chemistry, 29(5), 1756-1762. https://doi.org/10.1021/acs.bioconjchem.8b00195