Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94

Sho Kato; Takayuki Motoyama; Yushi Futamura; Masakazu Uramoto; Toshihiko Nogawa; Toshiaki Hayashi; Hiroshi Hirota; Akira Tanaka; Naoko Takahashi-Ando; Takashi Kamakura; Hiroyuki Osada

doi:10.1080/09168451.2020.1725419

Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94

Sho Kato, Takayuki Motoyama, Yushi Futamura, Masakazu Uramoto, Toshihiko Nogawa, Toshiaki Hayashi, Hiroshi Hirota, Akira Tanaka, Naoko Takahashi-Ando, Takashi Kamakura, Hiroyuki Osada^*

^*Corresponding author for this work

Research output: Contribution to journal › Comment/debate › peer-review

9 Citations (Scopus)

Abstract

We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97–94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.

Original language	English
Pages (from-to)	1303-1307
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	84
Issue number	6
DOIs	https://doi.org/10.1080/09168451.2020.1725419
Publication status	Published - 2020 Jun 2
Externally published	Yes

Keywords

Natural products
PKS-NRPS
biological activity
biosynthesis
malaria

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2020.1725419

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Kato, S, Motoyama, T, Futamura, Y, Uramoto, M, Nogawa, T, Hayashi, T, Hirota, H, Tanaka, A, Takahashi-Ando, N, Kamakura, T & Osada, H 2020, 'Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94', Bioscience, Biotechnology and Biochemistry, vol. 84, no. 6, pp. 1303-1307. https://doi.org/10.1080/09168451.2020.1725419

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title = "Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94",

abstract = "We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97–94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.",

keywords = "Natural products, PKS-NRPS, biological activity, biosynthesis, malaria",

author = "Sho Kato and Takayuki Motoyama and Yushi Futamura and Masakazu Uramoto and Toshihiko Nogawa and Toshiaki Hayashi and Hiroshi Hirota and Akira Tanaka and Naoko Takahashi-Ando and Takashi Kamakura and Hiroyuki Osada",

note = "Funding Information: This study was supported by grants from JSPS KAKENHI Grant Numbers [JP17H06412, JP17K7742, JP17K7784, and JP18H03945]. We would like to thank Ms. Harumi Aono, Dr. Rachael Uson-Lopez, Ms. Emiko Sanada, and Dr. Motoko Uchida for their technical support. Publisher Copyright: {\textcopyright} 2020, {\textcopyright} 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.",

year = "2020",

month = jun,

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doi = "10.1080/09168451.2020.1725419",

language = "English",

volume = "84",

pages = "1303--1307",

journal = "Bioscience, Biotechnology and Biochemistry",

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TY - JOUR

T1 - Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94

AU - Kato, Sho

AU - Motoyama, Takayuki

AU - Futamura, Yushi

AU - Uramoto, Masakazu

AU - Nogawa, Toshihiko

AU - Hayashi, Toshiaki

AU - Hirota, Hiroshi

AU - Tanaka, Akira

AU - Takahashi-Ando, Naoko

AU - Kamakura, Takashi

AU - Osada, Hiroyuki

N1 - Funding Information: This study was supported by grants from JSPS KAKENHI Grant Numbers [JP17H06412, JP17K7742, JP17K7784, and JP18H03945]. We would like to thank Ms. Harumi Aono, Dr. Rachael Uson-Lopez, Ms. Emiko Sanada, and Dr. Motoko Uchida for their technical support. Publisher Copyright: © 2020, © 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

PY - 2020/6/2

Y1 - 2020/6/2

N2 - We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97–94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.

AB - We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97–94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.

KW - Natural products

KW - PKS-NRPS

KW - biological activity

KW - biosynthesis

KW - malaria

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DO - 10.1080/09168451.2020.1725419

M3 - Comment/debate

C2 - 32043422

AN - SCOPUS:85079400161

SN - 0916-8451

VL - 84

SP - 1303

EP - 1307

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94

Abstract

Keywords

ASJC Scopus subject areas

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