Biotechnological production of vanillin using immobilized enzymes

Toshiki Furuya, Mari Kuroiwa, Kuniki Kino

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Vanillin is an important and popular plant flavor, but the amount of this compound available from plant sources is very limited. Biotechnological methods have high potential for vanillin production as an alternative to extraction from plant sources. Here, we report a new approach using immobilized enzymes for the production of vanillin. The recently discovered oxygenase Cso2 has coenzyme-independent catalytic activity for the conversion of isoeugenol and 4-vinylguaiacol to vanillin. Immobilization of Cso2 on Sepabeads EC-EA anion-exchange carrier conferred enhanced operational stability enabling repetitive use. This immobilized Cso2 catalyst allowed 6.8 mg yield of vanillin from isoeugenol through ten reaction cycles at a 1 mL scale. The coenzyme-independent decarboxylase Fdc, which has catalytic activity for the conversion of ferulic acid to 4-vinylguaiacol, was also immobilized on Sepabeads EC-EA. We demonstrated that the immobilized Fdc and Cso2 enabled the cascade synthesis of vanillin from ferulic acid via 4-vinylguaiacol with repetitive use of the catalysts. This study is the first example of biotechnological production of vanillin using immobilized enzymes, a process that provides new possibilities for vanillin production.

    Original languageEnglish
    Pages (from-to)25-28
    Number of pages4
    JournalJournal of Biotechnology
    Volume243
    DOIs
    Publication statusPublished - 2017 Feb 10

    Keywords

    • Biocatalysis
    • Cascade reaction
    • Ferulic acid
    • Immobilized enzyme
    • Vanillin

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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