Abstract
An understanding of the local changes in cerebral oxygen content accompanying functional brain activation is critical for making a valid signal interpretation of hemodynamic-based functional brain imaging. However, spatiotemporal relations between changes in tissue partial pressure of oxygen (PO2) and induced neural activity remain incompletely understood. To characterize the local PO2 response to the given neural activity, the authors simultaneously measured tissue PO2 and neural activity in the identical region of guinea pig auditory cortex with an oxygen microelectrode (tip < 10 μm) and optical recording with voltage-sensitive dye (RH 795). In addition, a laser displacement gauge and a laser-Doppler flowmeter were used to monitor the spatial displacement and regional cerebral blood flow, respectively, in the PO2 measurement region. In the activated region, tissue PO2 initially decreased during the ∼3-seconds after the onset of acoustic stimuli, and then increased during the next ∼5 seconds. Such biphasic changes are consistently found in cortical layers I to IV. In addition, amplitude of the biphasic change was closely related to detected peak height of the optical signal changes. The results suggest that the initial decrease in tissue PO2 is coupled to the induced neural activity and depends on response time of local increase in cerebral blood flow.
| Original language | English |
|---|---|
| Pages (from-to) | 1075-1084 |
| Number of pages | 10 |
| Journal | Journal of Cerebral Blood Flow and Metabolism |
| Volume | 23 |
| Issue number | 9 |
| Publication status | Published - 2003 Sep 1 |
| Externally published | Yes |
Fingerprint
Keywords
- Functional brain imaging
- Neural activity
- Oxygen microelectrode
- Tissue pO
ASJC Scopus subject areas
- Endocrinology
- Neuroscience(all)
- Endocrinology, Diabetes and Metabolism
Cite this
Biphasic changes in tissue partial pressure of oxygen closely related to localized neural activity in guinea pig auditory cortex. / Masamoto, Kazuto; Omura, Tetsuro; Takizawa, Naosada; Kobayashi, Hirosuke; Katura, Takusige; Maki, Atsushi; Kawaguchi, Hideo; Tanishita, Kazuo.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 23, No. 9, 01.09.2003, p. 1075-1084.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biphasic changes in tissue partial pressure of oxygen closely related to localized neural activity in guinea pig auditory cortex
AU - Masamoto, Kazuto
AU - Omura, Tetsuro
AU - Takizawa, Naosada
AU - Kobayashi, Hirosuke
AU - Katura, Takusige
AU - Maki, Atsushi
AU - Kawaguchi, Hideo
AU - Tanishita, Kazuo
PY - 2003/9/1
Y1 - 2003/9/1
N2 - An understanding of the local changes in cerebral oxygen content accompanying functional brain activation is critical for making a valid signal interpretation of hemodynamic-based functional brain imaging. However, spatiotemporal relations between changes in tissue partial pressure of oxygen (PO2) and induced neural activity remain incompletely understood. To characterize the local PO2 response to the given neural activity, the authors simultaneously measured tissue PO2 and neural activity in the identical region of guinea pig auditory cortex with an oxygen microelectrode (tip < 10 μm) and optical recording with voltage-sensitive dye (RH 795). In addition, a laser displacement gauge and a laser-Doppler flowmeter were used to monitor the spatial displacement and regional cerebral blood flow, respectively, in the PO2 measurement region. In the activated region, tissue PO2 initially decreased during the ∼3-seconds after the onset of acoustic stimuli, and then increased during the next ∼5 seconds. Such biphasic changes are consistently found in cortical layers I to IV. In addition, amplitude of the biphasic change was closely related to detected peak height of the optical signal changes. The results suggest that the initial decrease in tissue PO2 is coupled to the induced neural activity and depends on response time of local increase in cerebral blood flow.
AB - An understanding of the local changes in cerebral oxygen content accompanying functional brain activation is critical for making a valid signal interpretation of hemodynamic-based functional brain imaging. However, spatiotemporal relations between changes in tissue partial pressure of oxygen (PO2) and induced neural activity remain incompletely understood. To characterize the local PO2 response to the given neural activity, the authors simultaneously measured tissue PO2 and neural activity in the identical region of guinea pig auditory cortex with an oxygen microelectrode (tip < 10 μm) and optical recording with voltage-sensitive dye (RH 795). In addition, a laser displacement gauge and a laser-Doppler flowmeter were used to monitor the spatial displacement and regional cerebral blood flow, respectively, in the PO2 measurement region. In the activated region, tissue PO2 initially decreased during the ∼3-seconds after the onset of acoustic stimuli, and then increased during the next ∼5 seconds. Such biphasic changes are consistently found in cortical layers I to IV. In addition, amplitude of the biphasic change was closely related to detected peak height of the optical signal changes. The results suggest that the initial decrease in tissue PO2 is coupled to the induced neural activity and depends on response time of local increase in cerebral blood flow.
KW - Functional brain imaging
KW - Neural activity
KW - Oxygen microelectrode
KW - Tissue pO
UR - http://www.scopus.com/inward/record.url?scp=0041835957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041835957&partnerID=8YFLogxK
M3 - Article
C2 - 12973024
AN - SCOPUS:0041835957
VL - 23
SP - 1075
EP - 1084
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 9
ER -