Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation

Brianna J. Klein, Uma M. Muthurajan, Marie Eve Lalonde, Matthew D. Gibson, Forest H. Andrews, Maggie Hepler, Shinichi Machida, Kezhi Yan, Hitoshi Kurumizaka, Michael G. Poirier, Jacques Côté, Karolin Luger, Tatiana G. Kutateladze

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acetyltransferase (HAT) complex, critical for normal developmental programs and implicated in acute leukemias. BRPF1 contains a unique assembly of zinc fingers, termed a PZP domain, the physiological role of which remains unclear. Here, we elucidate the structure-function relationship of this novel epigenetic reader and detail the biological and mechanistic consequences of its interaction with nucleosomes. PZP has a globular architecture and forms a 2:1 stoichiometry complex with the nucleosome, bivalently interacting with histone H3 and DNA. This binding impacts the nucleosome dynamics, shifting the DNA unwrapping/rewrapping equilibrium toward the unwrapped state and increasing DNA accessibility. We demonstrate that the DNA-binding function of the BRPF1 PZP domain is required for the MOZ-BRPF1-ING5-hEaf6 HAT complex to be recruited to chromatin and to acetylate nucleosomal histones. Our findings reveal a novel link between chromatin dynamics and MOZ-mediated acetylation.

    Original languageEnglish
    Pages (from-to)472-484
    Number of pages13
    JournalNucleic Acids Research
    Volume44
    Issue number1
    DOIs
    Publication statusPublished - 2016 Jan 8

    Fingerprint

    Nucleosomes
    Acetylation
    Fingers
    Chromatin
    Histone Acetyltransferases
    DNA
    Histones
    Zinc Fingers
    Epigenomics
    Leukemia

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Klein, B. J., Muthurajan, U. M., Lalonde, M. E., Gibson, M. D., Andrews, F. H., Hepler, M., ... Kutateladze, T. G. (2016). Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation. Nucleic Acids Research, 44(1), 472-484. https://doi.org/10.1093/nar/gkv1321

    Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation. / Klein, Brianna J.; Muthurajan, Uma M.; Lalonde, Marie Eve; Gibson, Matthew D.; Andrews, Forest H.; Hepler, Maggie; Machida, Shinichi; Yan, Kezhi; Kurumizaka, Hitoshi; Poirier, Michael G.; Côté, Jacques; Luger, Karolin; Kutateladze, Tatiana G.

    In: Nucleic Acids Research, Vol. 44, No. 1, 08.01.2016, p. 472-484.

    Research output: Contribution to journalArticle

    Klein, BJ, Muthurajan, UM, Lalonde, ME, Gibson, MD, Andrews, FH, Hepler, M, Machida, S, Yan, K, Kurumizaka, H, Poirier, MG, Côté, J, Luger, K & Kutateladze, TG 2016, 'Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation', Nucleic Acids Research, vol. 44, no. 1, pp. 472-484. https://doi.org/10.1093/nar/gkv1321
    Klein, Brianna J. ; Muthurajan, Uma M. ; Lalonde, Marie Eve ; Gibson, Matthew D. ; Andrews, Forest H. ; Hepler, Maggie ; Machida, Shinichi ; Yan, Kezhi ; Kurumizaka, Hitoshi ; Poirier, Michael G. ; Côté, Jacques ; Luger, Karolin ; Kutateladze, Tatiana G. / Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation. In: Nucleic Acids Research. 2016 ; Vol. 44, No. 1. pp. 472-484.
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