Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black

Tin-Tin-Win-Shwe, Shoji Yamamoto, Sohel Ahmed, Masaki Kakeyama, Takahiro Kobayashi, Hidekazu Fujimaki

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Ambient air ultrafine particles (UFPs) have gained enormous attention to many researchers with recent evidence showing them to have more hazardous effects on human health than larger ambient particles. Studies focusing the possibility of effects on brain are quite limited. To examine the effect of ultrafine carbon black (ufCB) on mice brain, we instilled 125 μg of 14 nm or 95 nm CB into the nostrils of 8-week-old male BALB/c mice, once a week for 4 weeks. Four hours after the last instillation, we collected olfactory bulb and hippocampus and detected the expression of cytokine and chemokine mRNA by quantitative real-time PCR method. In this study, we found the induction of proinflammatory cytokines (interleukin-1β and tumor necrosis factor-α and chemokines (monocyte chemoattractant protein-1/CCL2, macrophage inflammatory protein-1α/CCL3), and monokine induced interferon-gamma/CXC chemokine ligand (CXCL9) mRNA in brain olfactory bulb, not in the hippocampus of mice instilled with 14 nm ufCB intranasally. We suggest that the intranasal instillation of ufCB may influence the brain immune function depending on their size. To our knowledge, this is the first study to demonstrate region-specific brain cytokine and chemokine mRNA-induction in mice triggered by intranasal instillation of specific-sized ufCB, in a physiologically relevant condition.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalToxicology Letters
Volume163
Issue number2
DOIs
Publication statusPublished - 2006 May 25
Externally publishedYes

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Soot
Chemokines
Brain
Cytokines
Messenger RNA
Olfactory Bulb
Hippocampus
Chemokine CXCL9
C Chemokines
Monokines
Macrophage Inflammatory Proteins
CXC Chemokines
Chemokine CCL2
Interleukin-1
Interferon-gamma
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Air
Research Personnel
Ultrafine

Keywords

  • Brain
  • Chemokines
  • Cytokines
  • Immune system
  • Intranasal instillation
  • Ultrafine particles

ASJC Scopus subject areas

  • Toxicology

Cite this

Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black. / Tin-Tin-Win-Shwe; Yamamoto, Shoji; Ahmed, Sohel; Kakeyama, Masaki; Kobayashi, Takahiro; Fujimaki, Hidekazu.

In: Toxicology Letters, Vol. 163, No. 2, 25.05.2006, p. 153-160.

Research output: Contribution to journalArticle

Tin-Tin-Win-Shwe ; Yamamoto, Shoji ; Ahmed, Sohel ; Kakeyama, Masaki ; Kobayashi, Takahiro ; Fujimaki, Hidekazu. / Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black. In: Toxicology Letters. 2006 ; Vol. 163, No. 2. pp. 153-160.
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