Brush biopsy of human oral mucosal epithelial cells as a quality control of the cell source for fabrication of transplantable epithelial cell sheets for regenerative medicine

Yoshiyuki Kasai, Hiroaki Sugiyama, Ryo Takagi, Makoto Kondo, Toshiyuki Owaki, Hideo Namiki, Teruo Okano, Naoya Takeda, Masayuki Yamato

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    Abstract

    Autologous oral mucosal epithelial cell sheets have been used for treating epithelial defects such as cornea and esophagus. The cell source of patients' oral mucosal epithelial cell sheet should be examined in normality because it has individual difference. In this study, oral mucosal epithelial cells were less invasively collected by brush biopsy from the buccal, gingival, labial, and palate mucosa of four healthy volunteer donors without anesthesia, and analyzed the keratin expressions by western blotting and the obtained results were compared with those by immunohistochemistry of each of the native tissues. All of the oral mucosal epithelial cells expressed keratin 4 (K4) and K13, which were mucosal stratified squamous epithelial cell markers. K1 and K10, keratinized epithelial cell markers, were also detected in keratinized tissues such as gingival and palate mucosa. The markers of epithelial basal cells such as p63 and K15 were not detected by brush biopsy-western blotting. Although this method does not include basal layers of oral mucosa, protein expressions of upper layer of lesion area are different from normal. Therefore, brush biopsy-western blotting was extremely less invasive and would contribute to quality control of the fabrication of autologous oral mucosal epithelial cell sheets.

    Original languageEnglish
    Pages (from-to)71-77
    Number of pages7
    JournalRegenerative Therapy
    Volume4
    DOIs
    Publication statusPublished - 2016 Jun 1

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    Keywords

    • Brush biopsy
    • Cell sheet
    • Human oral mucosal epithelial cell
    • Keratin expression
    • Quality control

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Developmental Biology
    • Biomaterials

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