Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination

Sarah Farmer, Eun Jin Erica Hong, Wing Kit Leung, Bilge Argunhan, Yaroslav Terentyev, Neil Humphryes, Hiroshi Toyoizumi, Hideo Tsubouchi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events. In contrast to previous studies where the function of Pch2 was implicated in the steps after meiotic double-strand breaks (DSBs) are formed, we present evidence that Pch2 is involved in meiotic DSB formation, the initiation step of meiotic recombination. The reduction of DSB formation caused by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background. The DSB reduction is further pronounced when pch2 is combined with a hypomorphic allele of SPO11. Interestingly, the level of DSB reduction is highly variable between chromosomes, with minimal impact on small chromosomes VI and III. We propose a model in which Pch2 ensures efficient formation of meiotic DSBs which is necessary for igniting the subsequent meiotic checkpoint responses that lead to proper differentiation of meiotic recombinants.

Original languageEnglish
Article numbere39724
JournalPLoS One
Volume7
Issue number6
DOIs
Publication statusPublished - 2012 Jun 22

Fingerprint

Saccharomycetales
Yeast
Genetic Recombination
Chromosomes
yeasts
chromosomes
mutants
Fungal Proteins
Meiosis
meiosis
Proteins
proteins
Alleles
alleles
mutation
Mutation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Farmer, S., Hong, E. J. E., Leung, W. K., Argunhan, B., Terentyev, Y., Humphryes, N., ... Tsubouchi, H. (2012). Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS One, 7(6), [e39724]. https://doi.org/10.1371/journal.pone.0039724

Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. / Farmer, Sarah; Hong, Eun Jin Erica; Leung, Wing Kit; Argunhan, Bilge; Terentyev, Yaroslav; Humphryes, Neil; Toyoizumi, Hiroshi; Tsubouchi, Hideo.

In: PLoS One, Vol. 7, No. 6, e39724, 22.06.2012.

Research output: Contribution to journalArticle

Farmer, S, Hong, EJE, Leung, WK, Argunhan, B, Terentyev, Y, Humphryes, N, Toyoizumi, H & Tsubouchi, H 2012, 'Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination', PLoS One, vol. 7, no. 6, e39724. https://doi.org/10.1371/journal.pone.0039724
Farmer, Sarah ; Hong, Eun Jin Erica ; Leung, Wing Kit ; Argunhan, Bilge ; Terentyev, Yaroslav ; Humphryes, Neil ; Toyoizumi, Hiroshi ; Tsubouchi, Hideo. / Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. In: PLoS One. 2012 ; Vol. 7, No. 6.
@article{fbc27bef0af64d00a50c4e5538941b08,
title = "Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination",
abstract = "Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events. In contrast to previous studies where the function of Pch2 was implicated in the steps after meiotic double-strand breaks (DSBs) are formed, we present evidence that Pch2 is involved in meiotic DSB formation, the initiation step of meiotic recombination. The reduction of DSB formation caused by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background. The DSB reduction is further pronounced when pch2 is combined with a hypomorphic allele of SPO11. Interestingly, the level of DSB reduction is highly variable between chromosomes, with minimal impact on small chromosomes VI and III. We propose a model in which Pch2 ensures efficient formation of meiotic DSBs which is necessary for igniting the subsequent meiotic checkpoint responses that lead to proper differentiation of meiotic recombinants.",
author = "Sarah Farmer and Hong, {Eun Jin Erica} and Leung, {Wing Kit} and Bilge Argunhan and Yaroslav Terentyev and Neil Humphryes and Hiroshi Toyoizumi and Hideo Tsubouchi",
year = "2012",
month = "6",
day = "22",
doi = "10.1371/journal.pone.0039724",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination

AU - Farmer, Sarah

AU - Hong, Eun Jin Erica

AU - Leung, Wing Kit

AU - Argunhan, Bilge

AU - Terentyev, Yaroslav

AU - Humphryes, Neil

AU - Toyoizumi, Hiroshi

AU - Tsubouchi, Hideo

PY - 2012/6/22

Y1 - 2012/6/22

N2 - Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events. In contrast to previous studies where the function of Pch2 was implicated in the steps after meiotic double-strand breaks (DSBs) are formed, we present evidence that Pch2 is involved in meiotic DSB formation, the initiation step of meiotic recombination. The reduction of DSB formation caused by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background. The DSB reduction is further pronounced when pch2 is combined with a hypomorphic allele of SPO11. Interestingly, the level of DSB reduction is highly variable between chromosomes, with minimal impact on small chromosomes VI and III. We propose a model in which Pch2 ensures efficient formation of meiotic DSBs which is necessary for igniting the subsequent meiotic checkpoint responses that lead to proper differentiation of meiotic recombinants.

AB - Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events. In contrast to previous studies where the function of Pch2 was implicated in the steps after meiotic double-strand breaks (DSBs) are formed, we present evidence that Pch2 is involved in meiotic DSB formation, the initiation step of meiotic recombination. The reduction of DSB formation caused by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background. The DSB reduction is further pronounced when pch2 is combined with a hypomorphic allele of SPO11. Interestingly, the level of DSB reduction is highly variable between chromosomes, with minimal impact on small chromosomes VI and III. We propose a model in which Pch2 ensures efficient formation of meiotic DSBs which is necessary for igniting the subsequent meiotic checkpoint responses that lead to proper differentiation of meiotic recombinants.

UR - http://www.scopus.com/inward/record.url?scp=84862671731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862671731&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0039724

DO - 10.1371/journal.pone.0039724

M3 - Article

C2 - 22745819

AN - SCOPUS:84862671731

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e39724

ER -