Buffers to suppress sodium dodecyl sulfate adsorption to polyethylene oxide for protein separation on capillary polymer electrophoresis

Keiko Sumitomo, Koichi Mayumi, Hiroyuki Minamikawa, Mitsutoshi Masuda, Toru Asahi, Toshimi Shimizu, Kohzo Ito, Yoshinori Yamaguchi

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    4 Citations (Scopus)

    Abstract

    Although polyethylene oxide (PEO) offers several advantages as a sieving polymer in SDS capillary polymer electrophoresis (SDS-CPE), solution properties of PEO cause deterioration in the electrophoresis because PEO in solution aggregates itself, degrades into smaller pieces, and forms polymer-micelle complexes with SDS. We examined protein separation on SDS-CPE with PEO as a sieving matrix in four individual buffer solutions: Tris-CHES, Tris-Gly, Tris-Tricine, and Tris-HCl buffers. The solution properties of PEO as a sieving matrix in those buffers were examined by dynamic light scattering (DLS) and by surface tension. Preferential SDS adsorption onto PEO disturbed protein-SDS complexation and impaired the protein separation efficiency. Substantial adsorption of SDS to PEO was particularly observed in Tris-Gly buffer. The Tris-CHES buffer prevented SDS from adsorbing onto the PEO. Only Tris-CHES buffer achieved separation of six proteins. This study demonstrated efficient protein separation on SDS-CPE with PEO.

    Original languageEnglish
    Pages (from-to)448-454
    Number of pages7
    JournalElectrophoresis
    Volume32
    Issue number3-4
    DOIs
    Publication statusPublished - 2011 Feb

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    Keywords

    • Capillary polymer electrophoresis
    • Polyethylene oxide
    • Polymer surfactant complexation
    • Protein separation

    ASJC Scopus subject areas

    • Biochemistry
    • Clinical Biochemistry

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