Ca2+ signaling regulated by an ATP-dependent autocrine mechanism in astrocytes

H. Shiga, T. Tojima, Etsuro Ito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Although the mechanisms of Ca2+ wave propagation in astrocytes induced by mechanical stimulation have been well studied, it is still not known how the [Ca2+]i increases in the stimulated cells. Here, we have analyzed the mechanisms of [Ca2+]i increase in single, isolated astrocytes. Our results showed that there was an autocrine mechanism of Ca2+ regulation mediated by ATP in mechanically stimulated astrocytes. This autocrine mechanism induced the activation of phospholipase C via a G-protein, resulting in Ca2+ release from intracellular Ca2+ stores. A second pathway mediating a [Ca2+]i increase was via a Ca2+ influx from the extracellular space, which, interestingly, suppressed an intracellular Ca2+ oscillation. These two different Ca2+ cascades are involved in signal transduction and may function separately during intercellular communication.

Original languageEnglish
Pages (from-to)2619-2622
Number of pages4
JournalNeuroReport
Volume12
Issue number12
Publication statusPublished - 2001 Aug 28
Externally publishedYes

Fingerprint

Astrocytes
Adenosine Triphosphate
Extracellular Space
Type C Phospholipases
GTP-Binding Proteins
Signal Transduction

Keywords

  • Calcium oscillation
  • Phospholipase C
  • Purine receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ca2+ signaling regulated by an ATP-dependent autocrine mechanism in astrocytes. / Shiga, H.; Tojima, T.; Ito, Etsuro.

In: NeuroReport, Vol. 12, No. 12, 28.08.2001, p. 2619-2622.

Research output: Contribution to journalArticle

Shiga, H. ; Tojima, T. ; Ito, Etsuro. / Ca2+ signaling regulated by an ATP-dependent autocrine mechanism in astrocytes. In: NeuroReport. 2001 ; Vol. 12, No. 12. pp. 2619-2622.
@article{59a5520323604fe0a9bd6506326dbf1c,
title = "Ca2+ signaling regulated by an ATP-dependent autocrine mechanism in astrocytes",
abstract = "Although the mechanisms of Ca2+ wave propagation in astrocytes induced by mechanical stimulation have been well studied, it is still not known how the [Ca2+]i increases in the stimulated cells. Here, we have analyzed the mechanisms of [Ca2+]i increase in single, isolated astrocytes. Our results showed that there was an autocrine mechanism of Ca2+ regulation mediated by ATP in mechanically stimulated astrocytes. This autocrine mechanism induced the activation of phospholipase C via a G-protein, resulting in Ca2+ release from intracellular Ca2+ stores. A second pathway mediating a [Ca2+]i increase was via a Ca2+ influx from the extracellular space, which, interestingly, suppressed an intracellular Ca2+ oscillation. These two different Ca2+ cascades are involved in signal transduction and may function separately during intercellular communication.",
keywords = "Calcium oscillation, Phospholipase C, Purine receptor",
author = "H. Shiga and T. Tojima and Etsuro Ito",
year = "2001",
month = "8",
day = "28",
language = "English",
volume = "12",
pages = "2619--2622",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - Ca2+ signaling regulated by an ATP-dependent autocrine mechanism in astrocytes

AU - Shiga, H.

AU - Tojima, T.

AU - Ito, Etsuro

PY - 2001/8/28

Y1 - 2001/8/28

N2 - Although the mechanisms of Ca2+ wave propagation in astrocytes induced by mechanical stimulation have been well studied, it is still not known how the [Ca2+]i increases in the stimulated cells. Here, we have analyzed the mechanisms of [Ca2+]i increase in single, isolated astrocytes. Our results showed that there was an autocrine mechanism of Ca2+ regulation mediated by ATP in mechanically stimulated astrocytes. This autocrine mechanism induced the activation of phospholipase C via a G-protein, resulting in Ca2+ release from intracellular Ca2+ stores. A second pathway mediating a [Ca2+]i increase was via a Ca2+ influx from the extracellular space, which, interestingly, suppressed an intracellular Ca2+ oscillation. These two different Ca2+ cascades are involved in signal transduction and may function separately during intercellular communication.

AB - Although the mechanisms of Ca2+ wave propagation in astrocytes induced by mechanical stimulation have been well studied, it is still not known how the [Ca2+]i increases in the stimulated cells. Here, we have analyzed the mechanisms of [Ca2+]i increase in single, isolated astrocytes. Our results showed that there was an autocrine mechanism of Ca2+ regulation mediated by ATP in mechanically stimulated astrocytes. This autocrine mechanism induced the activation of phospholipase C via a G-protein, resulting in Ca2+ release from intracellular Ca2+ stores. A second pathway mediating a [Ca2+]i increase was via a Ca2+ influx from the extracellular space, which, interestingly, suppressed an intracellular Ca2+ oscillation. These two different Ca2+ cascades are involved in signal transduction and may function separately during intercellular communication.

KW - Calcium oscillation

KW - Phospholipase C

KW - Purine receptor

UR - http://www.scopus.com/inward/record.url?scp=0035964095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035964095&partnerID=8YFLogxK

M3 - Article

C2 - 11522936

AN - SCOPUS:0035964095

VL - 12

SP - 2619

EP - 2622

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 12

ER -