Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150Glued and DNC-2/dynamitin

Masahiro Terasawa, Mika Toya, Fumio Motegi, Miyeko Mana, Kuniaki Nakamura, Asako Sugimoto

Research output: Contribution to journalArticle

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Abstract

Dynactin is a multisubunit protein complex required for the activity of cytoplasmic dynein. In Caenorhabditis elegans, although 10 of the 11 dynactin subunits were identified based on the sequence similarities to their orthologs, the p24/p22 subunit has not been detected in the genome. Here, we demonstrate that DNC-3 (W10G11.20) is the functional counterpart of the p24/p22 subunit in C. elegans. RNAi phenotypes and subcellular localization of DNC-3 in early C. elegans embryos were nearly identical to those of the known dynactin components. All other dynactin subunits were co-immunoprecipitated with DNC-3, indicating that DNC-3 is a core component of dynactin. Furthermore, the overall secondary structure of DNC-3 resembles to those of the mammalian and yeast p24/p22. We found that DNC-3 is required for the localization of the DNC-1/p150Glued and DNC-2/dynamitin, the two components of the projection arm of dynactin, to the nuclear envelope of meiotic nuclei in the adult gonad. Moreover, DNC-3 physically interacted with DNC-1 and DNC-2 and significantly enhanced the binding ability between DNC-1 and DNC-2 in vitro. These results suggest that DNC-3 is essential for the formation of the projection arm subcomplex of dynactin.

Original languageEnglish
Pages (from-to)1145-1157
Number of pages13
JournalGenes to Cells
Volume15
Issue number11
DOIs
Publication statusPublished - 2010 Nov
Externally publishedYes

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Caenorhabditis elegans
Cytoplasmic Dyneins
Nuclear Envelope
Gonads
RNA Interference
Dynactin Complex
Embryonic Structures
Yeasts
Genome
Phenotype
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150Glued and DNC-2/dynamitin. / Terasawa, Masahiro; Toya, Mika; Motegi, Fumio; Mana, Miyeko; Nakamura, Kuniaki; Sugimoto, Asako.

In: Genes to Cells, Vol. 15, No. 11, 11.2010, p. 1145-1157.

Research output: Contribution to journalArticle

Terasawa, Masahiro ; Toya, Mika ; Motegi, Fumio ; Mana, Miyeko ; Nakamura, Kuniaki ; Sugimoto, Asako. / Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150Glued and DNC-2/dynamitin. In: Genes to Cells. 2010 ; Vol. 15, No. 11. pp. 1145-1157.
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