Calexcitin-like immunoreactivity in the pond snail Lymnaea stagnalis

Dai Hatakeyama, Satoko Inamura, Etsuro Ito, Manabu Sakakibara, Thomas J. Nelson, Daniel L. Alkon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Calexcitin (CE) is a low molecular weight Ca2+- and guanosine triphosphate- binding protein, which is phosphorylated during associative learning in both vertebrates and invertebrates. The purpose of this study was to determine the presence of CE in the central nervous system (CNS) of the pond snail Lymnaea stagnalis, which can acquire classical and operant conditioning. Immunoblotting of CE showed that the anti-CE antibody prepared from squid can detect Lymnaea CE. In the cerebral ganglia, CE-like immunoreactivity was exhibited in two pairs of cell clusters that receive taste signals from the superior or median lip nerves. In both pedal ganglia, CE-like immunoreactivity was detected in 1-4 cell of the PeA clusters, which are involved in the withdrawal response. Our results therefore showed that CE is involved in the feeding and withdrawal neural networks, suggesting that CE may function in associative learning of feeding and withdrawal behavior in L. stagnalis.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalNeuroscience Research Communications
Volume35
Issue number1
DOIs
Publication statusPublished - 2004 Jul
Externally publishedYes

Fingerprint

Lymnaea
Snails
Ganglia
Learning
Operant Conditioning
Decapodiformes
Classical Conditioning
Median Nerve
Feeding Behavior
Invertebrates
Lip
Guanosine Triphosphate
Immunoblotting
Vertebrates
Foot
Anti-Idiotypic Antibodies
Carrier Proteins
Central Nervous System
Molecular Weight

Keywords

  • Ca-binding protein
  • GTP-binding protein
  • Mollusc
  • Withdrawal

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hatakeyama, D., Inamura, S., Ito, E., Sakakibara, M., Nelson, T. J., & Alkon, D. L. (2004). Calexcitin-like immunoreactivity in the pond snail Lymnaea stagnalis. Neuroscience Research Communications, 35(1), 32-40. https://doi.org/10.1002/nrc.20017

Calexcitin-like immunoreactivity in the pond snail Lymnaea stagnalis. / Hatakeyama, Dai; Inamura, Satoko; Ito, Etsuro; Sakakibara, Manabu; Nelson, Thomas J.; Alkon, Daniel L.

In: Neuroscience Research Communications, Vol. 35, No. 1, 07.2004, p. 32-40.

Research output: Contribution to journalArticle

Hatakeyama, D, Inamura, S, Ito, E, Sakakibara, M, Nelson, TJ & Alkon, DL 2004, 'Calexcitin-like immunoreactivity in the pond snail Lymnaea stagnalis', Neuroscience Research Communications, vol. 35, no. 1, pp. 32-40. https://doi.org/10.1002/nrc.20017
Hatakeyama, Dai ; Inamura, Satoko ; Ito, Etsuro ; Sakakibara, Manabu ; Nelson, Thomas J. ; Alkon, Daniel L. / Calexcitin-like immunoreactivity in the pond snail Lymnaea stagnalis. In: Neuroscience Research Communications. 2004 ; Vol. 35, No. 1. pp. 32-40.
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