Calreticulin is present in the acrosome of spermatids of rat testis

Masahisa Nakamura, Yuichi Michikawa, Tadashi Baba, Shoichi Okinaga, Kiyoshi Arai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have already succeeded in purifying a calcium-binding protein (CalBP) from rat spermatogenic cells [Nakamura et al., Biochem. Biophys. Res. Commun., 176(1991)1358]. In this study, the location of this protein within rat testis was examined, using a rabbit antisera for this protein. The antigen was localized on the developing acrosomes during spermiogenesis. The NH2-terminal amino acid sequence obtained for rat CalBP was identical to that of calreticulin obtained for the skeletal muscle of mice and closely resembled that for rabbit calreticulin. On the immunoblot analysis, the purified rat CalBP reacted with an antibody raised against rabbit skeletal muscle calreticulin. The results indicate that calreticulin is present in the acrosome of spermatids of rat testes.

Original languageEnglish
Pages (from-to)668-673
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
Publication statusPublished - 1992 Jul 31
Externally publishedYes

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Calreticulin
Acrosome
Spermatids
Testis
Rats
Calcium-Binding Proteins
Rabbits
Muscle
Skeletal Muscle
Spermatogenesis
Immune Sera
Amino Acid Sequence
Proteins
Antigens
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calreticulin is present in the acrosome of spermatids of rat testis. / Nakamura, Masahisa; Michikawa, Yuichi; Baba, Tadashi; Okinaga, Shoichi; Arai, Kiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 2, 31.07.1992, p. 668-673.

Research output: Contribution to journalArticle

Nakamura, Masahisa ; Michikawa, Yuichi ; Baba, Tadashi ; Okinaga, Shoichi ; Arai, Kiyoshi. / Calreticulin is present in the acrosome of spermatids of rat testis. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 2. pp. 668-673.
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