Abstract
Mutations of the Glu76 residue of canonical histone H2B are frequently found in cancer cells. However, it is quite mysterious how a single amino acid substitution in one of the multiple H2B genes affects cell fate. Here we found that the H2B E76K mutation, in which Glu76 is replaced by Lys (E76K), distorted the interface between H2B and H4 in the nucleosome, as revealed by the crystal structure and induced nucleosome instability in vivo and in vitro. Exogenous production of the H2B E76K mutant robustly enhanced the colony formation ability of the expressing cells, indicating that the H2B E76K mutant has the potential to promote oncogenic transformation in the presence of wild-type H2B. We found that other cancer-associated mutations of histones, H3.1 E97K and H2A.Z.1 R80C, also induced nucleosome instability. Interestingly, like the H2B E76K mutant, the H3.1 E97K mutant was minimally incorporated into chromatin in cells, but it enhanced the colony formation ability. In contrast, the H2A.Z.1 R80C mutant was incorporated into chromatin in cells, and had minor effects on the colony formation ability of the cells. These characteristics of histones with cancer-associated mutations may provide important information toward understanding how the mutations promote cancer progression.
| Original language | English |
|---|---|
| Pages (from-to) | 10007-10018 |
| Number of pages | 12 |
| Journal | Nucleic Acids Research |
| Volume | 46 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - 2018 Nov 2 |
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Cite this
Cancer-associated mutations of histones H2B, H3.1 and H2A.Z.1 affect the structure and stability of the nucleosome. / Arimura, Yasuhiro; Ikura, Masae; Fujita, Risa; Noda, Mamiko; Kobayashi, Wataru; Horikoshi, Naoki; Sun, Jiying; Shi, Lin; Kusakabe, Masayuki; Harata, Masahiko; Ohkawa, Yasuyuki; Tashiro, Satoshi; Kimura, Hiroshi; Ikura, Tsuyoshi; Kurumizaka, Hitoshi.
In: Nucleic Acids Research, Vol. 46, No. 19, 02.11.2018, p. 10007-10018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cancer-associated mutations of histones H2B, H3.1 and H2A.Z.1 affect the structure and stability of the nucleosome
AU - Arimura, Yasuhiro
AU - Ikura, Masae
AU - Fujita, Risa
AU - Noda, Mamiko
AU - Kobayashi, Wataru
AU - Horikoshi, Naoki
AU - Sun, Jiying
AU - Shi, Lin
AU - Kusakabe, Masayuki
AU - Harata, Masahiko
AU - Ohkawa, Yasuyuki
AU - Tashiro, Satoshi
AU - Kimura, Hiroshi
AU - Ikura, Tsuyoshi
AU - Kurumizaka, Hitoshi
PY - 2018/11/2
Y1 - 2018/11/2
N2 - Mutations of the Glu76 residue of canonical histone H2B are frequently found in cancer cells. However, it is quite mysterious how a single amino acid substitution in one of the multiple H2B genes affects cell fate. Here we found that the H2B E76K mutation, in which Glu76 is replaced by Lys (E76K), distorted the interface between H2B and H4 in the nucleosome, as revealed by the crystal structure and induced nucleosome instability in vivo and in vitro. Exogenous production of the H2B E76K mutant robustly enhanced the colony formation ability of the expressing cells, indicating that the H2B E76K mutant has the potential to promote oncogenic transformation in the presence of wild-type H2B. We found that other cancer-associated mutations of histones, H3.1 E97K and H2A.Z.1 R80C, also induced nucleosome instability. Interestingly, like the H2B E76K mutant, the H3.1 E97K mutant was minimally incorporated into chromatin in cells, but it enhanced the colony formation ability. In contrast, the H2A.Z.1 R80C mutant was incorporated into chromatin in cells, and had minor effects on the colony formation ability of the cells. These characteristics of histones with cancer-associated mutations may provide important information toward understanding how the mutations promote cancer progression.
AB - Mutations of the Glu76 residue of canonical histone H2B are frequently found in cancer cells. However, it is quite mysterious how a single amino acid substitution in one of the multiple H2B genes affects cell fate. Here we found that the H2B E76K mutation, in which Glu76 is replaced by Lys (E76K), distorted the interface between H2B and H4 in the nucleosome, as revealed by the crystal structure and induced nucleosome instability in vivo and in vitro. Exogenous production of the H2B E76K mutant robustly enhanced the colony formation ability of the expressing cells, indicating that the H2B E76K mutant has the potential to promote oncogenic transformation in the presence of wild-type H2B. We found that other cancer-associated mutations of histones, H3.1 E97K and H2A.Z.1 R80C, also induced nucleosome instability. Interestingly, like the H2B E76K mutant, the H3.1 E97K mutant was minimally incorporated into chromatin in cells, but it enhanced the colony formation ability. In contrast, the H2A.Z.1 R80C mutant was incorporated into chromatin in cells, and had minor effects on the colony formation ability of the cells. These characteristics of histones with cancer-associated mutations may provide important information toward understanding how the mutations promote cancer progression.
UR - http://www.scopus.com/inward/record.url?scp=85056260677&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056260677&partnerID=8YFLogxK
U2 - 10.1093/nar/gky661
DO - 10.1093/nar/gky661
M3 - Article
C2 - 30053102
AN - SCOPUS:85056260677
VL - 46
SP - 10007
EP - 10018
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 19
ER -