Carbocisteine normalizes the viscous property of mucus through regulation of fucosylated and sialylated sugar chain on airway mucins

Yuji Ishibashi, Goh Takayama, Yoshio Inouye, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Almost all of fucose and sialic acid in mucus are found on the mucus glycoproteins (mucins), and these sugar components on mucins are known to be associated with the viscous property of mucus. We have reported some aspects of carbocisteine, a mucoregulatory drug, correcting fucose and sialic acid contents in mucus. At present, carbocisteine's expectorant action of airway mucus is postulated to involve - the regulation of fucose and sialic acid contents on mucins. However little information is available about the relationship between the viscosity and sugar contents on mucins when treated with carbocisteine. To investigate further the mechanism behind the action of carbocisteine, the present study prepared MUC5AC fusion protein which has tandem repeat regions associated with MUC5AC, and evaluated the effects of carbocisteine on tumor necrosis factor (TNF)-α-induced increases of mucus viscosity and sialyl-Lewis x-epitopes antigen, an antigen which consists of fucosylated and sialylated sugar chains on the MUC5AC fusion proteins. Carbocisteine inhibited the TNF-α-induced increases of the viscosity and sialyl-Lewis x-epitopes on MUC5AC fusion protein. These findings suggest that carbocisteine may normalize the viscosity of mucus through "balancing" of fucose and sialic acid contents on airway mucins.

Original languageEnglish
Pages (from-to)226-228
Number of pages3
JournalEuropean Journal of Pharmacology
Volume641
Issue number2-3
DOIs
Publication statusPublished - 2010 Sep
Externally publishedYes

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Keywords

  • Carbocisteine
  • MUC5AC fusion protein
  • Sialyl-Lewis x
  • TNF-α
  • Viscosity

ASJC Scopus subject areas

  • Pharmacology

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