Carbonylated proteins in aging and exercise: Immunoblot approaches

S. Goto, A. Nakamura, Zsolt Radak, H. Nakamoto, R. Takahashi, K. Yasuda, Y. Sakurai, N. Ishii

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Protein carbonyls were studied in aging and exercise by immunoblot followed by one- or two-dimensional polyacrylamide gel electrophoresis using antibodies against 2,4-dinitrophenylhydrazones. Proteins of rat kidneys exhibited significant age-related increase in the amount of carbonyl while those of the brain and liver did not. Major carbonylated proteins in the kidney included serum albumin. In nematodes in which protein carbonyls increased with age, one of the carbonylated proteins was identified as vitellogenin, an egg-yolk protein. A possible biological significance of this protein present in abundance even after egg-laying stages is discussed in terms of protection against oxidative stress. Exhaustive exercise induced significant increase in the carbonylation of selected but unidentified proteins in the lung. This oxidative stress might be caused by xanthine oxidase in this tissue and hypoxanthine derived from ATP-depleted muscles. Exercise at high altitude caused higher carbonylation of the skeletal muscle proteins, most notably a protein likely to be actin, than that at sea level but no significant difference was observed in lipid peroxidation. These studies emphasize the value of immunoblot analysis of tissue protein carbonyls in a variety of situations where oxidative stress is likely involved.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalMechanisms of Ageing and Development
Volume107
Issue number3
DOIs
Publication statusPublished - 1999 Mar 15
Externally publishedYes

Fingerprint

Proteins
Oxidative Stress
Kidney
Vitellogenins
Egg Proteins
Hypoxanthine
Muscle Proteins
Xanthine Oxidase
Electrophoresis, Gel, Two-Dimensional
Serum Albumin
Oceans and Seas
Lipid Peroxidation
Ovum
Actins
Skeletal Muscle
Adenosine Triphosphate
Muscles
Lung
Antibodies
Liver

Keywords

  • Aging
  • Exercise
  • Nematode
  • Protein carbonyl
  • Protein oxidation
  • Vitellogenin

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

Cite this

Goto, S., Nakamura, A., Radak, Z., Nakamoto, H., Takahashi, R., Yasuda, K., ... Ishii, N. (1999). Carbonylated proteins in aging and exercise: Immunoblot approaches. Mechanisms of Ageing and Development, 107(3), 245-253. https://doi.org/10.1016/S0047-6374(98)00133-X

Carbonylated proteins in aging and exercise : Immunoblot approaches. / Goto, S.; Nakamura, A.; Radak, Zsolt; Nakamoto, H.; Takahashi, R.; Yasuda, K.; Sakurai, Y.; Ishii, N.

In: Mechanisms of Ageing and Development, Vol. 107, No. 3, 15.03.1999, p. 245-253.

Research output: Contribution to journalArticle

Goto, S, Nakamura, A, Radak, Z, Nakamoto, H, Takahashi, R, Yasuda, K, Sakurai, Y & Ishii, N 1999, 'Carbonylated proteins in aging and exercise: Immunoblot approaches', Mechanisms of Ageing and Development, vol. 107, no. 3, pp. 245-253. https://doi.org/10.1016/S0047-6374(98)00133-X
Goto, S. ; Nakamura, A. ; Radak, Zsolt ; Nakamoto, H. ; Takahashi, R. ; Yasuda, K. ; Sakurai, Y. ; Ishii, N. / Carbonylated proteins in aging and exercise : Immunoblot approaches. In: Mechanisms of Ageing and Development. 1999 ; Vol. 107, No. 3. pp. 245-253.
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