Carboxyl end-specific monoclonal antibodies to amyloid β protein (Aβ) subtypes (Aβ40 and Aβ42(43)) differentiate Aβ in senile plaques and amyloid angiopathy in brains of aged cynomolgus monkeys

Shin'ichiro Nakamura, Akira Tamaoka, Naoya Sawamura, Shin'ichi Shoji, Hiroyuki Nakayama, Fumiko Ono, Ippei Sakakibara, Yasuhiro Yoshikawa, Hiroshi Mori, Naoaki Goto, Kunio Doi

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31 Citations (Scopus)

Abstract

Senile plaques (SPs) and cerebral amyloid angiopathy (CAA) in the brains of five aged (20-26 years old) cynomolgus monkeys were investigated immunohistochemically using two monoclonal antibodies (anti-Aβ 40 (BA27) and anti-Aβ 42(43) (BC05)) that can differentiate the carboxyl termini of amyloid β protein (Aβ) subtypes. In four of five animals, all types of SPs (i.e. diffuse, primitive, and classical plaques; DPs, PPs, and CPs, respectively) were identified by BC05. However, BA27 did not label DPs and stained only about one third of PPs and CPs, mainly labeling granular structures and cored portions, respectively. In CAA, lesions of cortical capillaries reacted to BC05 in four of five cases, but rarely and weakly to BA27 in two of five cases. On the other hand, lesions of parenchymal and meningeal arterioles were stained by both BA27 and BC05. These staining profiles of SPs in cynomolgus monkeys correspond well to those in humans, although there are two remarkable features in cynomolgus monkeys. First, BA27 stained PPs associated with granular structures. Secondly, capillary Aβ reacted intensely to BC05 but only slightly to BA27. Despite these unique features, the results suggest that aged cynomolgus monkeys can be used to investigate the pathogenesis of Aβ deposition in SPs and CAA.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalNeuroscience Letters
Volume201
Issue number2
DOIs
Publication statusPublished - 1995 Dec 8
Externally publishedYes

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Keywords

  • Alzheimer's disease
  • Amyloid angiopathy
  • Amyloid β protein
  • Cynomolgus monkey
  • Senile plaque

ASJC Scopus subject areas

  • Neuroscience(all)

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