Catalytic function of the mycobacterial binuclear iron monooxygenase in acetone metabolism

Toshiki Furuya, Tomomi Nakao, Kuniki Kino

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Mycobacteria such as Mycobacterium smegmatis strain mc2155 and Mycobacterium goodii strain 12523 are able to grow on acetone and use it as a source of carbon and energy. We previously demonstrated by gene deletion analysis that the mimABCD gene cluster, which encodes a binuclear iron monooxygenase, plays an essential role in acetone metabolism in these mycobacteria. In the present study, we determined the catalytic function of MimABCD in acetone metabolism. Whole-cell assays were performed using Escherichia coli cells expressing the MimABCD complex. When the recombinant E. coli cells were incubated with acetone, a product was detected by gas chromatography (GC) analysis. Based on the retention time and the gas chromatography-mass spectrometry (GC-MS) spectrum, the reaction product was identified as acetol (hydroxyacetone). The recombinant E.coli cells produced 1.02 mM of acetol from acetone within 24 h. Furthermore, we demonstrated that MimABCD also was able to convert methylethylketone (2-butanone) to 1-hydroxy-2-butanone. Although it has long been known that microorganisms such as mycobacteria metabolize acetone via acetol, this study provides the first biochemical evidence for the existence of a microbial enzyme that catalyses the conversion of acetone to acetol.

    Original languageEnglish
    Article numberfnv136
    JournalFEMS Microbiology Letters
    Volume362
    Issue number19
    DOIs
    Publication statusPublished - 2015 Aug 31

    Fingerprint

    Acetone
    Iron
    Mycobacterium
    Escherichia coli
    Mycobacterium smegmatis
    Gene Deletion
    Multigene Family
    Mixed Function Oxygenases
    acetone monooxygenase
    Gas Chromatography
    Gas Chromatography-Mass Spectrometry
    Carbon
    acetol
    Enzymes

    Keywords

    • Acetol
    • acetone
    • MimABCD
    • monooxygenase
    • mycobacteria
    • oxidation

    ASJC Scopus subject areas

    • Microbiology
    • Genetics
    • Molecular Biology

    Cite this

    Catalytic function of the mycobacterial binuclear iron monooxygenase in acetone metabolism. / Furuya, Toshiki; Nakao, Tomomi; Kino, Kuniki.

    In: FEMS Microbiology Letters, Vol. 362, No. 19, fnv136, 31.08.2015.

    Research output: Contribution to journalArticle

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