Abstract
During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins, segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. This attachment allows rapid poleward movement of the bound chromosome. Subsequent attachment of the sister kinetochore to MTs growing from the other centrosome results in the bi-orientation of the chromosome, in which interactions between kinetochores and the plus ends of MTs are formed and stabilized. These processes ensure alignment of chromosomes during metaphase and their correct segregation during anaphase. Although many proteins constituting the kinetochore have been identified and extensively studied, the signalling responsible for MT capture and stabilization is unclear. Small GTPases of the Rho family regulate cell morphogenesis by organizing the actin cytoskeleton and regulating MT alignment and stabilization. We now show that one member of this family, Cdc42, and its effector, mDia3, regulate MT attachment to kinetochores.
| Original language | English |
|---|---|
| Pages (from-to) | 767-771 |
| Number of pages | 5 |
| Journal | Nature |
| Volume | 428 |
| Issue number | 6984 |
| DOIs | |
| Publication status | Published - 2004 Apr 15 |
| Externally published | Yes |
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Cdc42 and mDia3 regulate microtubule attachment to kinetochores. / Yasuda, Shingo; Oceguera-Yanez, Fabian; Kato, Takayuki; Okamoto, Muneo; Yonemura, Shigenobu; Terada, Yasuhiko; Ishizaki, Toshimasa; Narumiya, Shuh.
In: Nature, Vol. 428, No. 6984, 15.04.2004, p. 767-771.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cdc42 and mDia3 regulate microtubule attachment to kinetochores
AU - Yasuda, Shingo
AU - Oceguera-Yanez, Fabian
AU - Kato, Takayuki
AU - Okamoto, Muneo
AU - Yonemura, Shigenobu
AU - Terada, Yasuhiko
AU - Ishizaki, Toshimasa
AU - Narumiya, Shuh
PY - 2004/4/15
Y1 - 2004/4/15
N2 - During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins, segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. This attachment allows rapid poleward movement of the bound chromosome. Subsequent attachment of the sister kinetochore to MTs growing from the other centrosome results in the bi-orientation of the chromosome, in which interactions between kinetochores and the plus ends of MTs are formed and stabilized. These processes ensure alignment of chromosomes during metaphase and their correct segregation during anaphase. Although many proteins constituting the kinetochore have been identified and extensively studied, the signalling responsible for MT capture and stabilization is unclear. Small GTPases of the Rho family regulate cell morphogenesis by organizing the actin cytoskeleton and regulating MT alignment and stabilization. We now show that one member of this family, Cdc42, and its effector, mDia3, regulate MT attachment to kinetochores.
AB - During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins, segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. This attachment allows rapid poleward movement of the bound chromosome. Subsequent attachment of the sister kinetochore to MTs growing from the other centrosome results in the bi-orientation of the chromosome, in which interactions between kinetochores and the plus ends of MTs are formed and stabilized. These processes ensure alignment of chromosomes during metaphase and their correct segregation during anaphase. Although many proteins constituting the kinetochore have been identified and extensively studied, the signalling responsible for MT capture and stabilization is unclear. Small GTPases of the Rho family regulate cell morphogenesis by organizing the actin cytoskeleton and regulating MT alignment and stabilization. We now show that one member of this family, Cdc42, and its effector, mDia3, regulate MT attachment to kinetochores.
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U2 - 10.1038/nature02452
DO - 10.1038/nature02452
M3 - Article
VL - 428
SP - 767
EP - 771
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6984
ER -