CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly

Naoyuki Okada, Takashi Toda, Masayuki Yamamoto, Masamitsu Sato

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    As cells transition from interphase to mitosis, the microtubule cytoskeleton is reorganized to form the mitotic spindle. In the closed mitosis of fission yeast, a microtubule-associated protein complex, Alp7-Alp14 (transforming acidic coiled-coil-tumor overexpressed gene), enters the nucleus upon mitotic entry and promotes spindle formation. However, how the complex is controlled to accumulate in the nucleus only during mitosis remains elusive. Here we demonstrate that Alp7-Alp14 is excluded from the nucleus during interphase using the nuclear export signal in Alp14 but is accumulated in the nucleus during mitosis through phosphorylation of Alp7 by the cyclin-dependent kinase (CDK). Five phosphorylation sites reside around the nuclear localization signal of Alp7, and the phosphodeficient alp7-5A mutant fails to accumulate in the nucleus during mitosis and exhibits partial spindle defects. Thus our results reveal one way that CDK regulates spindle assembly at mitotic entry: CDK phosphorylates the Alp7-Alp14 complex to localize it to the nucleus.

    Original languageEnglish
    Pages (from-to)1969-1982
    Number of pages14
    JournalMolecular Biology of the Cell
    Volume25
    Issue number13
    DOIs
    Publication statusPublished - 2014 Jul 1

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    Cyclin-Dependent Kinases
    Mitosis
    Microtubules
    Phosphorylation
    Interphase
    Nuclear Export Signals
    Nuclear Localization Signals
    Spindle Apparatus
    Microtubule-Associated Proteins
    Schizosaccharomyces
    Cytoskeleton
    Genes
    Neoplasms

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

    Cite this

    CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly. / Okada, Naoyuki; Toda, Takashi; Yamamoto, Masayuki; Sato, Masamitsu.

    In: Molecular Biology of the Cell, Vol. 25, No. 13, 01.07.2014, p. 1969-1982.

    Research output: Contribution to journalArticle

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