Cdk5 is required for multipolar-to-bipolar transition during neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex

Toshio Ohshima, Motoyuki Hirasawa, Hidenori Tabata, Tetsuji Mutoh, Tomoko Adachi, Hiromi Suzuki, Keiko Saruta, Takuji Iwasato, Shigeyoshi Itohara, Mistuhiro Hashimoto, Kazunori Nakajima, Masaharu Ogawa, Ashok B. Kulkarni, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The mammalian cerebral cortex consists of six layers that are generated via coordinated neuronal migration during the embryonic period. Recent studies identified specific phases of radial migration of cortical neurons. After the final division, neurons transform from a multipolar to a bipolar shape within the subventricular zone-intermediate zone (SVZ-IZ) and then migrate along radial glial fibres. Mice lacking Cdk5 exhibit abnormal corticogenesis owing to neuronal migration defects. When we introduced GFP into migrating neurons at E14.5 by in utero electroporation, we observed migrating neurons in wild-type but not in Cdk5-/- embryos after 3-4 days. Introduction of the dominant-negative form of Cdk5 into the wild-type migrating neurons confirmed specific impairment of the multipolar-to-bipolar transition within the SVZ-IZ in a cell-autonomous manner. Cortex-specific Cdk5 conditional knockout mice showed inverted layering of the cerebral cortex and the layer V and callosal neurons, but not layer VI neurons, had severely impaired dendritic morphology. The amount of the dendritic protein Map2 was decreased in the cerebral cortex of Cdk5-deficient mice, and the axonal trajectory of cortical neurons within the cortex was also abnormal. These results indicate that Cdk5 is required for proper multipolar-to-bipolar transition, and a deficiency of Cdk5 results in abnormal morphology of pyramidal neurons. In addition, proper radial neuronal migration generates an inside-out pattern of cerebral cortex formation and normal axonal trajectories of cortical pyramidal neurons.

Original languageEnglish
Pages (from-to)2273-2282
Number of pages10
JournalDevelopment
Volume134
Issue number12
DOIs
Publication statusPublished - 2007 Jun 1
Externally publishedYes

Keywords

  • Cdk5
  • Cerebral cortex
  • Map2 (Mtap2)
  • Mouse
  • Neuronal migration

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Ohshima, T., Hirasawa, M., Tabata, H., Mutoh, T., Adachi, T., Suzuki, H., Saruta, K., Iwasato, T., Itohara, S., Hashimoto, M., Nakajima, K., Ogawa, M., Kulkarni, A. B., & Mikoshiba, K. (2007). Cdk5 is required for multipolar-to-bipolar transition during neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex. Development, 134(12), 2273-2282. https://doi.org/10.1242/dev.02854