The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts. The amino acid identities in the protein from C. elegans compared with those of bovine mitochondria and Eschericia coli are 29.5% and 24.0%, respectively. The C. elegans sequence was classified as a long EF-Ts (ca. 280 amino acids long) similar to peptides from mammalian mitochondria and eubacteria other than Thermus and cyanobacteria (except Spirulina platensis), rather than short EF- Ts (ca. 200 amino acids long) as those of Thermus, cyanobacteria and plastids.
|Number of pages||6|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|Publication status||Published - 1997 Jul 17|
- Elongation factor Ts
- RT-PCR (Caenorhabditis elegans)
ASJC Scopus subject areas
- Structural Biology