cDNA sequence of a translational elongation factor Ts homologue from Caenorhabditis elegans

Mitochondrial factor-specific features found in the nematode homologue peptide

Yoh Ichi Watanabe, Kiyoshi Kita, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts. The amino acid identities in the protein from C. elegans compared with those of bovine mitochondria and Eschericia coli are 29.5% and 24.0%, respectively. The C. elegans sequence was classified as a long EF-Ts (ca. 280 amino acids long) similar to peptides from mammalian mitochondria and eubacteria other than Thermus and cyanobacteria (except Spirulina platensis), rather than short EF- Ts (ca. 200 amino acids long) as those of Thermus, cyanobacteria and plastids.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1353
Issue number1
DOIs
Publication statusPublished - 1997 Jul 17
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Complementary DNA
Mitochondria
Thermus
Amino Acids
Peptides
Cyanobacteria
Caenorhabditis elegans Proteins
Spirulina
Plastids
Amino Acid Sequence
Bacteria
elongation factor Ts

Keywords

  • Elongation factor Ts
  • Mitochondrion
  • RT-PCR (Caenorhabditis elegans)
  • Translation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

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abstract = "The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts. The amino acid identities in the protein from C. elegans compared with those of bovine mitochondria and Eschericia coli are 29.5{\%} and 24.0{\%}, respectively. The C. elegans sequence was classified as a long EF-Ts (ca. 280 amino acids long) similar to peptides from mammalian mitochondria and eubacteria other than Thermus and cyanobacteria (except Spirulina platensis), rather than short EF- Ts (ca. 200 amino acids long) as those of Thermus, cyanobacteria and plastids.",
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T1 - cDNA sequence of a translational elongation factor Ts homologue from Caenorhabditis elegans

T2 - Mitochondrial factor-specific features found in the nematode homologue peptide

AU - Watanabe, Yoh Ichi

AU - Kita, Kiyoshi

AU - Ueda, Takuya

AU - Watanabe, Kimitsuna

PY - 1997/7/17

Y1 - 1997/7/17

N2 - The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts. The amino acid identities in the protein from C. elegans compared with those of bovine mitochondria and Eschericia coli are 29.5% and 24.0%, respectively. The C. elegans sequence was classified as a long EF-Ts (ca. 280 amino acids long) similar to peptides from mammalian mitochondria and eubacteria other than Thermus and cyanobacteria (except Spirulina platensis), rather than short EF- Ts (ca. 200 amino acids long) as those of Thermus, cyanobacteria and plastids.

AB - The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts. The amino acid identities in the protein from C. elegans compared with those of bovine mitochondria and Eschericia coli are 29.5% and 24.0%, respectively. The C. elegans sequence was classified as a long EF-Ts (ca. 280 amino acids long) similar to peptides from mammalian mitochondria and eubacteria other than Thermus and cyanobacteria (except Spirulina platensis), rather than short EF- Ts (ca. 200 amino acids long) as those of Thermus, cyanobacteria and plastids.

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KW - Translation

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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