CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo

Katsuhiro Sawasato, Ryo Sato, Hanako Nishikawa, Naoki Iimura, Yuki Kamemoto, Kohki Fujikawa, Toshiyuki Yamaguchi, Yutetsu Kuruma, Yasushi Tamura, Toshiya Endo, Takuya Ueda, Keiko Shimamoto, Ken ichi Nishiyama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

MPIase is a glycolipid that is involved in membrane protein integration. Despite evaluation of its functions in vitro, the lack of information on MPIase biosynthesis hampered verification of its involvement in vivo. In this study, we found that depletion of CdsA, a CDP-diacylglycerol synthase, caused not only a defect in phospholipid biosynthesis but also MPIase depletion with accumulation of the precursors of both membrane protein M13 coat protein and secretory protein OmpA. Yeast Tam41p, a mitochondrial CDP-diacylglycerol synthase, suppressed the defect in phospholipid biosynthesis, but restored neither MPIase biosynthesis, precursor processing, nor cell growth, indicating that MPIase is essential for membrane protein integration and therefore for cell growth. Consistently, we observed a severe defect in protein integration into MPIase-depleted membrane vesicles in vitro. Thus, the function of MPIase as a factor involved in protein integration was proven in vivo as well as in vitro. Moreover, Cds1p, a eukaryotic CdsA homologue, showed a potential for MPIase biosynthesis. From these results, we speculate the presence of a eukaryotic MPIase homologue.

Original languageEnglish
Article number1372
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1
Externally publishedYes

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Glycolipids
Diacylglycerol Cholinephosphotransferase
Membrane Proteins
Phospholipids
Capsid Proteins
Growth
Proteins
Yeasts
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Sawasato, K., Sato, R., Nishikawa, H., Iimura, N., Kamemoto, Y., Fujikawa, K., ... Nishiyama, K. I. (2019). CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo. Scientific reports, 9(1), [1372]. https://doi.org/10.1038/s41598-018-37809-8

CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo. / Sawasato, Katsuhiro; Sato, Ryo; Nishikawa, Hanako; Iimura, Naoki; Kamemoto, Yuki; Fujikawa, Kohki; Yamaguchi, Toshiyuki; Kuruma, Yutetsu; Tamura, Yasushi; Endo, Toshiya; Ueda, Takuya; Shimamoto, Keiko; Nishiyama, Ken ichi.

In: Scientific reports, Vol. 9, No. 1, 1372, 01.12.2019.

Research output: Contribution to journalArticle

Sawasato, K, Sato, R, Nishikawa, H, Iimura, N, Kamemoto, Y, Fujikawa, K, Yamaguchi, T, Kuruma, Y, Tamura, Y, Endo, T, Ueda, T, Shimamoto, K & Nishiyama, KI 2019, 'CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo', Scientific reports, vol. 9, no. 1, 1372. https://doi.org/10.1038/s41598-018-37809-8
Sawasato, Katsuhiro ; Sato, Ryo ; Nishikawa, Hanako ; Iimura, Naoki ; Kamemoto, Yuki ; Fujikawa, Kohki ; Yamaguchi, Toshiyuki ; Kuruma, Yutetsu ; Tamura, Yasushi ; Endo, Toshiya ; Ueda, Takuya ; Shimamoto, Keiko ; Nishiyama, Ken ichi. / CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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