Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds

Keiichi Imato; Kazuho Nagata; Rina Watanabe; Naoya Takeda

doi:10.1039/c9tb02958c

Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds

Keiichi Imato, Kazuho Nagata, Rina Watanabe, Naoya Takeda^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.

Original language	English
Pages (from-to)	2393-2399
Number of pages	7
Journal	Journal of Materials Chemistry B
Volume	8
Issue number	12
DOIs	https://doi.org/10.1039/c9tb02958c
Publication status	Published - 2020 Mar 28

ASJC Scopus subject areas

Chemistry(all)
Biomedical Engineering
Materials Science(all)

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title = "Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds",

abstract = "Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.",

author = "Keiichi Imato and Kazuho Nagata and Rina Watanabe and Naoya Takeda",

note = "Funding Information: K. I. gratefully acknowledges the financial support received from the Casio Science Promotion Foundation. This work was also partially supported by the Izumi Science and Technology Foundation (H27-J-172) (N.T.). The authors thank Tokyo Women{\textquoteright}s Medical University for the size exclusion chromatography, UV-vis absorption, and contact angle measurements. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

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doi = "10.1039/c9tb02958c",

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AU - Imato, Keiichi

AU - Nagata, Kazuho

AU - Watanabe, Rina

AU - Takeda, Naoya

N1 - Funding Information: K. I. gratefully acknowledges the financial support received from the Casio Science Promotion Foundation. This work was also partially supported by the Izumi Science and Technology Foundation (H27-J-172) (N.T.). The authors thank Tokyo Women’s Medical University for the size exclusion chromatography, UV-vis absorption, and contact angle measurements. Publisher Copyright: © 2020 The Royal Society of Chemistry.

PY - 2020/3/28

Y1 - 2020/3/28

N2 - Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.

AB - Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.

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Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds

Abstract

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