Cell attachment area of rat mesenchymal stem cells correlates with their osteogenic differentiation level on substrates without osteoconductive property

Shogo Hashimoto, Mayu Yasunaga, Motohiro Hirose, Masayuki Kakehata, Hidehiko Yashiro, Atsushi Yamazaki, Atsuo Ito

Research output: Contribution to journalArticle

Abstract

Cell morphology is related to proliferation and differentiation. We previously reported that cell attachment area of rat mesenchymal stem cells (MSCs) is negatively correlated with their osteogenic differentiation level on osteoconductive hydroxyapatite (HAp) with various microstructures. In this study, the correlation between the cell attachment area and osteogenic differentiation level was investigated on substrates without osteoconductive property using tissue culture polystyrene (TCPS), and 3 mol% yttria-stabilized zirconia (3Y-TZP) with or without surface periodic microstructures. It was found that the osteogenic differentiation level after 3 weeks of culture increased with a decrease in cell attachment area after 3 h of culture. The square of the correlation coefficient between cell attachment area and osteocalcin secretion content was 0.845 among the three types of substrates. Thus, the negative correlation between cell attachment area and differentiation level is confirmed even when cultured on substrates without osteoconductive property. These findings suggest that the correlation between the cell attachment area of rat MSCs and osteogenic differentiation level could also apply to various types of substrate, regardless of osteoconductive property.

Original languageEnglish
Pages (from-to)1081-1086
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume525
Issue number4
DOIs
Publication statusPublished - 2020 May 14

Keywords

  • Cell attachment area
  • Cell morphology
  • Mesenchymal stem cell
  • Osteoconductive property
  • Osteogenic differentiation level
  • Zirconia

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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