Cell cycle and size sorting of mammalian cells using a microfluidic device

Satoshi Migita, Kei Funakoshi, Daiju Tsuya, Tomohiko Yamazaki, Akiyoshi Taniguchi, Yoshimasa Sugimoto, Nobutaka Hanagata, Toshiyuki Ikoma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Microfluidic devices can sort viable mammalian cells by size. In this study, we investigated size-based sorting of cells using flow splitting microfluidic devices based on hydrodynamic filtration for noninvasive cell cycle synchronization. Two different types of mammalian cell lines, HepG2 (human hepatocellular liver carcinoma cell line) and NIH/3T3 (mouse embryonic fibroblast cell line) were sorted by microfluidic device and its DNA contents were analyzed. Our results showed that a microfluidic device can synchronize the cell cycle after size separation. The damage-free separation of living cells in different phases of the cell cycle represents a potentially promising technology for the investigation of gene transfection and gene expression.

Original languageEnglish
Pages (from-to)657-660
Number of pages4
JournalAnalytical Methods
Volume2
Issue number6
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

Fingerprint

Sorting
Microfluidics
Cells
Size separation
Fibroblasts
Gene expression
Liver
Synchronization
DNA
Hydrodynamics
Genes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Migita, S., Funakoshi, K., Tsuya, D., Yamazaki, T., Taniguchi, A., Sugimoto, Y., ... Ikoma, T. (2010). Cell cycle and size sorting of mammalian cells using a microfluidic device. Analytical Methods, 2(6), 657-660. https://doi.org/10.1039/c0ay00039f

Cell cycle and size sorting of mammalian cells using a microfluidic device. / Migita, Satoshi; Funakoshi, Kei; Tsuya, Daiju; Yamazaki, Tomohiko; Taniguchi, Akiyoshi; Sugimoto, Yoshimasa; Hanagata, Nobutaka; Ikoma, Toshiyuki.

In: Analytical Methods, Vol. 2, No. 6, 06.2010, p. 657-660.

Research output: Contribution to journalArticle

Migita, S, Funakoshi, K, Tsuya, D, Yamazaki, T, Taniguchi, A, Sugimoto, Y, Hanagata, N & Ikoma, T 2010, 'Cell cycle and size sorting of mammalian cells using a microfluidic device', Analytical Methods, vol. 2, no. 6, pp. 657-660. https://doi.org/10.1039/c0ay00039f
Migita, Satoshi ; Funakoshi, Kei ; Tsuya, Daiju ; Yamazaki, Tomohiko ; Taniguchi, Akiyoshi ; Sugimoto, Yoshimasa ; Hanagata, Nobutaka ; Ikoma, Toshiyuki. / Cell cycle and size sorting of mammalian cells using a microfluidic device. In: Analytical Methods. 2010 ; Vol. 2, No. 6. pp. 657-660.
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