Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast

Imène B. Bouhlel, Midori Ohta, Adeline Mayeux, Nicole Bordes, Florent Dingli, Jérôme Boulanger, Guilhem Velve Casquillas, Damarys Loew, Phong T. Tran, Masamitsu Sato, Anne Paoletti

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Spindle pole biogenesis and segregation are tightly coordinated to produce a bipolar mitotic spindle. In yeasts, the spindle pole body (SPB) half-bridge composed of Sfi1 and Cdc31 duplicates to promote the biogenesis of a second SPB. Sfi1 accumulates at the half-bridge in two phases in Schizosaccharomyces pombe, from anaphase to early septation and throughout G2 phase. We found that the function of Sfi1-Cdc31 in SPB duplication is accomplished before septation ends and G2 accumulation starts. Thus, Sfi1 early accumulation at mitotic exit might correspond to half-bridge duplication. We further show that Cdc31 phosphorylation on serine 15 in a Cdk1 (encoded by cdc2) consensus site is required for the dissociation of a significant pool of Sfi1 from the bridge and timely segregation of SPBs at mitotic onset. This suggests that the Cdc31 N-terminus modulates the stability of Sfi1-Cdc31 arrays in fission yeast, and impacts on the timing of establishment of spindle bipolarity.

    Original languageEnglish
    Pages (from-to)1481-1493
    Number of pages13
    JournalJournal of Cell Science
    Volume128
    Issue number8
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Spindle Pole Bodies
    Schizosaccharomyces
    Cell Cycle Checkpoints
    Spindle Poles
    Anaphase
    Spindle Apparatus
    G2 Phase
    Serine
    Yeasts
    Phosphorylation

    Keywords

    • Cell cycle
    • Centrosome
    • Mitotic spindle
    • SPB

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Bouhlel, I. B., Ohta, M., Mayeux, A., Bordes, N., Dingli, F., Boulanger, J., ... Paoletti, A. (2015). Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast. Journal of Cell Science, 128(8), 1481-1493. https://doi.org/10.1242/jcs.159657

    Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast. / Bouhlel, Imène B.; Ohta, Midori; Mayeux, Adeline; Bordes, Nicole; Dingli, Florent; Boulanger, Jérôme; Casquillas, Guilhem Velve; Loew, Damarys; Tran, Phong T.; Sato, Masamitsu; Paoletti, Anne.

    In: Journal of Cell Science, Vol. 128, No. 8, 2015, p. 1481-1493.

    Research output: Contribution to journalArticle

    Bouhlel, IB, Ohta, M, Mayeux, A, Bordes, N, Dingli, F, Boulanger, J, Casquillas, GV, Loew, D, Tran, PT, Sato, M & Paoletti, A 2015, 'Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast', Journal of Cell Science, vol. 128, no. 8, pp. 1481-1493. https://doi.org/10.1242/jcs.159657
    Bouhlel, Imène B. ; Ohta, Midori ; Mayeux, Adeline ; Bordes, Nicole ; Dingli, Florent ; Boulanger, Jérôme ; Casquillas, Guilhem Velve ; Loew, Damarys ; Tran, Phong T. ; Sato, Masamitsu ; Paoletti, Anne. / Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast. In: Journal of Cell Science. 2015 ; Vol. 128, No. 8. pp. 1481-1493.
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