Cell delivery using micropatterned ultra-thin polymeric films

Yoshihiro Mori; Toshinori Fujie; Nobuhiro Nagai; Matsuhiko Nishizawa; Ali Khademhosseini; Toshiaki Abe; Hirokazu Kaji

doi:10.11239/jsmbe.52.O-192

Cell delivery using micropatterned ultra-thin polymeric films

Yoshihiro Mori, Toshinori Fujie, Nobuhiro Nagai, Matsuhiko Nishizawa, Ali Khademhosseini, Toshiaki Abe, Hirokazu Kaji

Waseda Institute for Advanced Study

Research output: Contribution to journal › Article

Abstract

We developed micropatterned polymeric ultra-thin films (nanosheets) consisting of biodegradable poly(lactic-co-glycolic acid) (PLGA) and magnetic nanoparticles (MNPs), on which monolayer of retinal pigment epithelial (RPE) cells were formed. The viability of RPE cells on the micropatterned nanosheets were evaluated along the syringe manipulation equipped with a clinical needle. Finally, subretinal injectability of the macropatterned nanosheets was demonstrated by using ex vivo swine eyes in terms of handleability and physical stability. The micropatterned nanosheets injectable by clinical syringe hold a great promise to transplant functional RPE cells minimum invasively.

Original language	English
Pages (from-to)	O-192-O-193
Journal	Transactions of Japanese Society for Medical and Biological Engineering
Volume	52
DOIs	https://doi.org/10.11239/jsmbe.52.O-192
Publication status	Published - 2014 Aug 17

Fingerprint

Keywords

Cell delivery
Nanosheet
PLGA
RPE

ASJC Scopus subject areas

Biomedical Engineering

Cite this

Mori, Y., Fujie, T., Nagai, N., Nishizawa, M., Khademhosseini, A., Abe, T., & Kaji, H. (2014). Cell delivery using micropatterned ultra-thin polymeric films. Transactions of Japanese Society for Medical and Biological Engineering, 52, O-192-O-193. https://doi.org/10.11239/jsmbe.52.O-192

@article{a21be0e1659f4566ba44d290e6c72473,

title = "Cell delivery using micropatterned ultra-thin polymeric films",

abstract = "We developed micropatterned polymeric ultra-thin films (nanosheets) consisting of biodegradable poly(lactic-co-glycolic acid) (PLGA) and magnetic nanoparticles (MNPs), on which monolayer of retinal pigment epithelial (RPE) cells were formed. The viability of RPE cells on the micropatterned nanosheets were evaluated along the syringe manipulation equipped with a clinical needle. Finally, subretinal injectability of the macropatterned nanosheets was demonstrated by using ex vivo swine eyes in terms of handleability and physical stability. The micropatterned nanosheets injectable by clinical syringe hold a great promise to transplant functional RPE cells minimum invasively.",

keywords = "Cell delivery, Nanosheet, PLGA, RPE",

author = "Yoshihiro Mori and Toshinori Fujie and Nobuhiro Nagai and Matsuhiko Nishizawa and Ali Khademhosseini and Toshiaki Abe and Hirokazu Kaji",

year = "2014",

month = aug

day = "17",

doi = "10.11239/jsmbe.52.O-192",

language = "English",

volume = "52",

pages = "O--192--O--193",

journal = "BME = Bio medical engineering / henshu, Nihon ME Gakkai",

issn = "1347-443X",

publisher = "Nihon M E Gakkai",

}

TY - JOUR

T1 - Cell delivery using micropatterned ultra-thin polymeric films

AU - Mori, Yoshihiro

AU - Fujie, Toshinori

AU - Nagai, Nobuhiro

AU - Nishizawa, Matsuhiko

AU - Khademhosseini, Ali

AU - Abe, Toshiaki

AU - Kaji, Hirokazu

PY - 2014/8/17

Y1 - 2014/8/17

N2 - We developed micropatterned polymeric ultra-thin films (nanosheets) consisting of biodegradable poly(lactic-co-glycolic acid) (PLGA) and magnetic nanoparticles (MNPs), on which monolayer of retinal pigment epithelial (RPE) cells were formed. The viability of RPE cells on the micropatterned nanosheets were evaluated along the syringe manipulation equipped with a clinical needle. Finally, subretinal injectability of the macropatterned nanosheets was demonstrated by using ex vivo swine eyes in terms of handleability and physical stability. The micropatterned nanosheets injectable by clinical syringe hold a great promise to transplant functional RPE cells minimum invasively.

AB - We developed micropatterned polymeric ultra-thin films (nanosheets) consisting of biodegradable poly(lactic-co-glycolic acid) (PLGA) and magnetic nanoparticles (MNPs), on which monolayer of retinal pigment epithelial (RPE) cells were formed. The viability of RPE cells on the micropatterned nanosheets were evaluated along the syringe manipulation equipped with a clinical needle. Finally, subretinal injectability of the macropatterned nanosheets was demonstrated by using ex vivo swine eyes in terms of handleability and physical stability. The micropatterned nanosheets injectable by clinical syringe hold a great promise to transplant functional RPE cells minimum invasively.

KW - Cell delivery

KW - Nanosheet

KW - PLGA

KW - RPE

UR - http://www.scopus.com/inward/record.url?scp=84939448021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939448021&partnerID=8YFLogxK

U2 - 10.11239/jsmbe.52.O-192

DO - 10.11239/jsmbe.52.O-192

M3 - Article

AN - SCOPUS:84939448021

VL - 52

SP - O-192-O-193

JO - BME = Bio medical engineering / henshu, Nihon ME Gakkai

JF - BME = Bio medical engineering / henshu, Nihon ME Gakkai

SN - 1347-443X

ER -

Access to Document

10.11239/jsmbe.52.O-192