Cell-free protein synthesis in a microfabricated reactor

T. Nojima, T. Fujii, K. Hosokawa, A. Yotsumoto, Shuichi Shoji, I. Endo

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Microbiochemical reactors having two inlet ports and one outlet port were fabricated on a silicon wafer by means of anisotropic etching in order to develop a parallel and automatic experimental system for cell-free translation. Using cell-free extract prepared from Escherichia coli, we tested the reactor for the translation of polyuridylic acid and MS2 phage RNA, and found that polypeptide and protein syntheses could be proceeded according to the genetic codes on the mRNAs. It indicates that the microfabricated reactor is useful for enzymatic reactions including complicated ones like cell-free translation. We also discuss the possibility of microsystems as advanced experimental tools for not only cell-free translation but also other various biochemical and biological research fields.

    Original languageEnglish
    Pages (from-to)13-17
    Number of pages5
    JournalBioprocess Engineering
    Volume22
    Issue number1
    Publication statusPublished - 2000

    Fingerprint

    Poly U
    Anisotropic etching
    Bacteriophages
    Microsystems
    Polypeptides
    RNA
    Silicon wafers
    Escherichia coli
    Levivirus
    RNA Phages
    Genetic Code
    Proteins
    Messenger RNA
    Peptides
    Acids
    Cell-Free System
    Silicon
    Cell Extracts
    Research

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Chemical Engineering(all)
    • Bioengineering

    Cite this

    Nojima, T., Fujii, T., Hosokawa, K., Yotsumoto, A., Shoji, S., & Endo, I. (2000). Cell-free protein synthesis in a microfabricated reactor. Bioprocess Engineering, 22(1), 13-17.

    Cell-free protein synthesis in a microfabricated reactor. / Nojima, T.; Fujii, T.; Hosokawa, K.; Yotsumoto, A.; Shoji, Shuichi; Endo, I.

    In: Bioprocess Engineering, Vol. 22, No. 1, 2000, p. 13-17.

    Research output: Contribution to journalArticle

    Nojima, T, Fujii, T, Hosokawa, K, Yotsumoto, A, Shoji, S & Endo, I 2000, 'Cell-free protein synthesis in a microfabricated reactor', Bioprocess Engineering, vol. 22, no. 1, pp. 13-17.
    Nojima T, Fujii T, Hosokawa K, Yotsumoto A, Shoji S, Endo I. Cell-free protein synthesis in a microfabricated reactor. Bioprocess Engineering. 2000;22(1):13-17.
    Nojima, T. ; Fujii, T. ; Hosokawa, K. ; Yotsumoto, A. ; Shoji, Shuichi ; Endo, I. / Cell-free protein synthesis in a microfabricated reactor. In: Bioprocess Engineering. 2000 ; Vol. 22, No. 1. pp. 13-17.
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