Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery

Hideaki Matsubayashi, Yutetsu Kuruma, Takuya Ueda

Research output: Contribution to journalArticle

6 Citations (Scopus)


The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalOrigins of Life and Evolution of Biospheres
Issue number4
Publication statusPublished - 2014 Dec 1
Externally publishedYes



  • Cell-free protein synthesis system
  • Liposome
  • Membrane protein
  • SecYEG translocon

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Space and Planetary Science

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