Cell-free translation system using phosphorothioate-containing mRNA.

T. Ueda, H. Tohda, N. Chikazumi, F. Eckstein, K. Watanabe

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphorothioate-containing RNAs were generated by transcription of template DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S) and T7 RNA polymerase. The substitution of mRNA by phosphorothioate increased the efficiency of protein synthesis by stabilizing the mRNAs in prokaryotic cell-free translation systems. The substituted mRNAs were also shown to be applicable to the continuous cell-free translation system developed by Spirin and coworkers.

Original languageEnglish
Pages (from-to)151-152
Number of pages2
JournalNucleic acids symposium series
Issue number25
Publication statusPublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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