Cell patterning and navigation of cell migration on caged cell-culturing substrate

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda

Research output: Contribution to conferencePaper

Abstract

Caged cell-culturing substrate is composed of a glass substrate immobilized with a monolayer having a photocleavable protecting group. This substrate can be activated for cell adhesion by illumination of light. By using this substrate, we have succeeded in micropatterning of cells corresponding to photomasks inserted at the location of the field diaphragm of a standard fluorescence microscope. In the present study, we evaluated the effect of terminal functional groups in caged cell-culturing substrates on photoactivation of cell adhesion without using fibronectin. In addition, we applied one of the substrates to navigate migration of single cells from their original pattern by illumination of light.

Original languageEnglish
Pages5197-5198
Number of pages2
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Fingerprint

Navigation
Substrates
Cell adhesion
Lighting
Photomasks
Diaphragms
Functional groups
Monolayers
Microscopes
Fluorescence
Cells
Glass

Keywords

  • Caged compounds
  • Cell adhesion
  • Cell migration
  • Light
  • Monolayer
  • Patterning

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Nakanishi, J., Kikuchi, Y., Takarada, T., Nakayama, H., Yamaguchi, K., & Maeda, M. (2005). Cell patterning and navigation of cell migration on caged cell-culturing substrate. 5197-5198. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.

Cell patterning and navigation of cell migration on caged cell-culturing substrate. / Nakanishi, Jun; Kikuchi, Yukiko; Takarada, Tohru; Nakayama, Hidekazu; Yamaguchi, Kazuo; Maeda, Mizuo.

2005. 5197-5198 Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.

Research output: Contribution to conferencePaper

Nakanishi, J, Kikuchi, Y, Takarada, T, Nakayama, H, Yamaguchi, K & Maeda, M 2005, 'Cell patterning and navigation of cell migration on caged cell-culturing substrate' Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan, 05/9/20 - 05/9/22, pp. 5197-5198.
Nakanishi J, Kikuchi Y, Takarada T, Nakayama H, Yamaguchi K, Maeda M. Cell patterning and navigation of cell migration on caged cell-culturing substrate. 2005. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.
Nakanishi, Jun ; Kikuchi, Yukiko ; Takarada, Tohru ; Nakayama, Hidekazu ; Yamaguchi, Kazuo ; Maeda, Mizuo. / Cell patterning and navigation of cell migration on caged cell-culturing substrate. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.2 p.
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