Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

Takahiro Arakawa, Yoshitaka Shirasaki, Daisuke Yamazaki, Takashi Funatsu, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

    Original languageEnglish
    Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    PublisherChemical and Biological Microsystems Society
    Pages215-217
    Number of pages3
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    Duration: 2008 Oct 122008 Oct 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    CitySan Diego, CA
    Period08/10/1208/10/16

    Fingerprint

    Microfluidics
    Needles
    Pneumatics
    Glass
    Mitochondria
    Cell membranes
    Substrates

    Keywords

    • Cell rupture
    • Lysosome
    • Microvalve
    • Mitochondria
    • Nano needle array
    • Organelle

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Arakawa, T., Shirasaki, Y., Yamazaki, D., Funatsu, T., & Shoji, S. (2008). Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 215-217). Chemical and Biological Microsystems Society.

    Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. / Arakawa, Takahiro; Shirasaki, Yoshitaka; Yamazaki, Daisuke; Funatsu, Takashi; Shoji, Shuichi.

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 215-217.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Arakawa, T, Shirasaki, Y, Yamazaki, D, Funatsu, T & Shoji, S 2008, Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 215-217, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
    Arakawa T, Shirasaki Y, Yamazaki D, Funatsu T, Shoji S. Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 215-217
    Arakawa, Takahiro ; Shirasaki, Yoshitaka ; Yamazaki, Daisuke ; Funatsu, Takashi ; Shoji, Shuichi. / Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 215-217
    @inproceedings{36013d7d21ff46c29a85b706ac62c1c1,
    title = "Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles",
    abstract = "In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.",
    keywords = "Cell rupture, Lysosome, Microvalve, Mitochondria, Nano needle array, Organelle",
    author = "Takahiro Arakawa and Yoshitaka Shirasaki and Daisuke Yamazaki and Takashi Funatsu and Shuichi Shoji",
    year = "2008",
    language = "English",
    pages = "215--217",
    booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",
    publisher = "Chemical and Biological Microsystems Society",

    }

    TY - GEN

    T1 - Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

    AU - Arakawa, Takahiro

    AU - Shirasaki, Yoshitaka

    AU - Yamazaki, Daisuke

    AU - Funatsu, Takashi

    AU - Shoji, Shuichi

    PY - 2008

    Y1 - 2008

    N2 - In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

    AB - In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

    KW - Cell rupture

    KW - Lysosome

    KW - Microvalve

    KW - Mitochondria

    KW - Nano needle array

    KW - Organelle

    UR - http://www.scopus.com/inward/record.url?scp=84902482350&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84902482350&partnerID=8YFLogxK

    M3 - Conference contribution

    AN - SCOPUS:84902482350

    SP - 215

    EP - 217

    BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

    PB - Chemical and Biological Microsystems Society

    ER -