Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

Takahiro Arakawa, Yoshitaka Shirasaki, Daisuke Yamazaki, Takashi Funatsu, Shuichi Shoji

Research output: Contribution to conferencePaper

Abstract

In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

Original languageEnglish
Pages215-217
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

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Keywords

  • Cell rupture
  • Lysosome
  • Microvalve
  • Mitochondria
  • Nano needle array
  • Organelle

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Arakawa, T., Shirasaki, Y., Yamazaki, D., Funatsu, T., & Shoji, S. (2008). Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles. 215-217. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.