Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro

Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, Takashi Ohki, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    56 Citations (Scopus)

    Abstract

    During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution of cell shape and actomyosin activity to the contractile ring assembly remains unclear. Here, we demonstrated in vitro that actin polymerization inside cell-sized spherical droplets induced the spontaneous formation of single ring-shaped actin bundles in the presence of bundling factors. Despite a lack of spatial regulatory signals, the rings always assembled at the equator to minimize the elastic energy of the bundles. Myosin promoted ring formation by the dynamic remodelling of actin networks, and an increase in the effective concentration of myosin triggered ring contraction. These results will help us understand how animal cells coordinate cell shape and actomyosin activities to direct cytokinesis.

    Original languageEnglish
    Pages (from-to)480-489
    Number of pages10
    JournalNature Cell Biology
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - 2015 Apr 30

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    Actomyosin
    Myosins
    Actins
    Cytokinesis
    Cell Shape
    Actin Cytoskeleton
    Polymerization
    Cell Division
    In Vitro Techniques
    Proteins

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro. / Miyazaki, Makito; Chiba, Masataka; Eguchi, Hiroki; Ohki, Takashi; Ishiwata, Shin'ichi.

    In: Nature Cell Biology, Vol. 17, No. 4, 30.04.2015, p. 480-489.

    Research output: Contribution to journalArticle

    Miyazaki, Makito ; Chiba, Masataka ; Eguchi, Hiroki ; Ohki, Takashi ; Ishiwata, Shin'ichi. / Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro. In: Nature Cell Biology. 2015 ; Vol. 17, No. 4. pp. 480-489.
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