Cellular localization of the constitutive β-glucosidase in Trichoderma viride

Shoji Usami, Kotaro Kirimura, Masatoshi Imura, Satoshi Morikawa

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The cellular localization of the constitutive β-glucosidase in the mycelia of Trichoderma viride was investigated by cell fractionation using disrupted mycelia and mycelial protoplasts. In 2-d-old mycelia, approximately 86% of the cellular enzyme activity was detected in the fraction containing cytosol, plasma membrane, and periplasm, and 14% in that containing cell wall. With incubation of the mycelia in a lytic enzyme solution, the β-glucosidase was released rapidly before protoplast formation was observed. After 4 h of incubation in the lytic enzyme solution, only 4% of β-glucosidase activity was detected in the protoplasts recovered. These results indicated that most of the constitutive β-glucosidase was on the cell surface, especially in the cell wall and periplasm.

    Original languageEnglish
    Pages (from-to)185-187
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume70
    Issue number3
    DOIs
    Publication statusPublished - 1990

    Fingerprint

    Glucosidases
    Trichoderma
    Mycelium
    Protoplasts
    Periplasm
    Cell Wall
    Enzymes
    Cells
    Cell Fractionation
    Enzyme activity
    Cell membranes
    Fractionation
    Cytosol
    Cell Membrane

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Cellular localization of the constitutive β-glucosidase in Trichoderma viride. / Usami, Shoji; Kirimura, Kotaro; Imura, Masatoshi; Morikawa, Satoshi.

    In: Journal of Fermentation and Bioengineering, Vol. 70, No. 3, 1990, p. 185-187.

    Research output: Contribution to journalArticle

    Usami, Shoji ; Kirimura, Kotaro ; Imura, Masatoshi ; Morikawa, Satoshi. / Cellular localization of the constitutive β-glucosidase in Trichoderma viride. In: Journal of Fermentation and Bioengineering. 1990 ; Vol. 70, No. 3. pp. 185-187.
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