Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization

S. Nomura, K. Ogami, K. Kawamura, I. Tsukamoto, Y. Kudo, Y. Kanakura, Y. Kitamura, H. Miyazaki, Takashi Kato

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The expression of thrombopoietin (TPO) mRNA is observed in several tissues, including liver, kidney, brain, skeletal muscle, intestine, spleen, and bone marrow. Among these organs, the highest expression of TPO mRNA is detected in the liver. We identified cells producing TPO by means of in situ hybridization of adult rat liver using digoxigenin-11-UTP-labeled cRNA probes. We found that the cells expressing TPO mRNA also expressed serum albumin mRNA. TPO mRNA was detected in parenchymal cells (hepatocytes) but not in non-parenchymal cells (including endothelial cells, epithelial cells, and so forth). To determine the location of TPO expression in embryogenesis, sections of fetal mice were further analyzed by in situ hybridization. TPO mRNA was detected only in hepatocytes of fetal liver, which was also the major site of hematopoiesis. The expression of TPO mRNA in fetal liver was observed from 12.5 days postcoitus. Northern blot analysis showed that mouse liver transcribed the same size of TPO mRNA in the fetus and in the adult. These results clearly demonstrate that hepatocytes are the primary site of TPO production in the liver from fetus to adult.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalExperimental Hematology
Volume25
Issue number7
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Thrombopoietin
In Situ Hybridization
Messenger RNA
Liver
Hepatocytes
Fetus
Digoxigenin
Complementary RNA
Uridine Triphosphate
Hematopoiesis
Serum Albumin
Northern Blotting
Intestines
Embryonic Development
Skeletal Muscle
Spleen
Endothelial Cells
Bone Marrow
Epithelial Cells
Kidney

Keywords

  • Embryo
  • Hepatocyte
  • In situ hybridization
  • Liver
  • Thrombopoetin

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Nomura, S., Ogami, K., Kawamura, K., Tsukamoto, I., Kudo, Y., Kanakura, Y., ... Kato, T. (1997). Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization. Experimental Hematology, 25(7), 565-572.

Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization. / Nomura, S.; Ogami, K.; Kawamura, K.; Tsukamoto, I.; Kudo, Y.; Kanakura, Y.; Kitamura, Y.; Miyazaki, H.; Kato, Takashi.

In: Experimental Hematology, Vol. 25, No. 7, 1997, p. 565-572.

Research output: Contribution to journalArticle

Nomura, S, Ogami, K, Kawamura, K, Tsukamoto, I, Kudo, Y, Kanakura, Y, Kitamura, Y, Miyazaki, H & Kato, T 1997, 'Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization', Experimental Hematology, vol. 25, no. 7, pp. 565-572.
Nomura S, Ogami K, Kawamura K, Tsukamoto I, Kudo Y, Kanakura Y et al. Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization. Experimental Hematology. 1997;25(7):565-572.
Nomura, S. ; Ogami, K. ; Kawamura, K. ; Tsukamoto, I. ; Kudo, Y. ; Kanakura, Y. ; Kitamura, Y. ; Miyazaki, H. ; Kato, Takashi. / Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization. In: Experimental Hematology. 1997 ; Vol. 25, No. 7. pp. 565-572.
@article{5b2da873ddda43c9bf55cb09c8daee9b,
title = "Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization",
abstract = "The expression of thrombopoietin (TPO) mRNA is observed in several tissues, including liver, kidney, brain, skeletal muscle, intestine, spleen, and bone marrow. Among these organs, the highest expression of TPO mRNA is detected in the liver. We identified cells producing TPO by means of in situ hybridization of adult rat liver using digoxigenin-11-UTP-labeled cRNA probes. We found that the cells expressing TPO mRNA also expressed serum albumin mRNA. TPO mRNA was detected in parenchymal cells (hepatocytes) but not in non-parenchymal cells (including endothelial cells, epithelial cells, and so forth). To determine the location of TPO expression in embryogenesis, sections of fetal mice were further analyzed by in situ hybridization. TPO mRNA was detected only in hepatocytes of fetal liver, which was also the major site of hematopoiesis. The expression of TPO mRNA in fetal liver was observed from 12.5 days postcoitus. Northern blot analysis showed that mouse liver transcribed the same size of TPO mRNA in the fetus and in the adult. These results clearly demonstrate that hepatocytes are the primary site of TPO production in the liver from fetus to adult.",
keywords = "Embryo, Hepatocyte, In situ hybridization, Liver, Thrombopoetin",
author = "S. Nomura and K. Ogami and K. Kawamura and I. Tsukamoto and Y. Kudo and Y. Kanakura and Y. Kitamura and H. Miyazaki and Takashi Kato",
year = "1997",
language = "English",
volume = "25",
pages = "565--572",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - Cellular localization of thrombopoietin mRNA in the liver by in situ hybridization

AU - Nomura, S.

AU - Ogami, K.

AU - Kawamura, K.

AU - Tsukamoto, I.

AU - Kudo, Y.

AU - Kanakura, Y.

AU - Kitamura, Y.

AU - Miyazaki, H.

AU - Kato, Takashi

PY - 1997

Y1 - 1997

N2 - The expression of thrombopoietin (TPO) mRNA is observed in several tissues, including liver, kidney, brain, skeletal muscle, intestine, spleen, and bone marrow. Among these organs, the highest expression of TPO mRNA is detected in the liver. We identified cells producing TPO by means of in situ hybridization of adult rat liver using digoxigenin-11-UTP-labeled cRNA probes. We found that the cells expressing TPO mRNA also expressed serum albumin mRNA. TPO mRNA was detected in parenchymal cells (hepatocytes) but not in non-parenchymal cells (including endothelial cells, epithelial cells, and so forth). To determine the location of TPO expression in embryogenesis, sections of fetal mice were further analyzed by in situ hybridization. TPO mRNA was detected only in hepatocytes of fetal liver, which was also the major site of hematopoiesis. The expression of TPO mRNA in fetal liver was observed from 12.5 days postcoitus. Northern blot analysis showed that mouse liver transcribed the same size of TPO mRNA in the fetus and in the adult. These results clearly demonstrate that hepatocytes are the primary site of TPO production in the liver from fetus to adult.

AB - The expression of thrombopoietin (TPO) mRNA is observed in several tissues, including liver, kidney, brain, skeletal muscle, intestine, spleen, and bone marrow. Among these organs, the highest expression of TPO mRNA is detected in the liver. We identified cells producing TPO by means of in situ hybridization of adult rat liver using digoxigenin-11-UTP-labeled cRNA probes. We found that the cells expressing TPO mRNA also expressed serum albumin mRNA. TPO mRNA was detected in parenchymal cells (hepatocytes) but not in non-parenchymal cells (including endothelial cells, epithelial cells, and so forth). To determine the location of TPO expression in embryogenesis, sections of fetal mice were further analyzed by in situ hybridization. TPO mRNA was detected only in hepatocytes of fetal liver, which was also the major site of hematopoiesis. The expression of TPO mRNA in fetal liver was observed from 12.5 days postcoitus. Northern blot analysis showed that mouse liver transcribed the same size of TPO mRNA in the fetus and in the adult. These results clearly demonstrate that hepatocytes are the primary site of TPO production in the liver from fetus to adult.

KW - Embryo

KW - Hepatocyte

KW - In situ hybridization

KW - Liver

KW - Thrombopoetin

UR - http://www.scopus.com/inward/record.url?scp=0030753987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030753987&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 565

EP - 572

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 7

ER -