Cep169, a novel microtubule plus-end-tracking centrosomal protein, binds to CDK5RAP2 and regulates microtubule stability

Yusuke Mori, Yoko Inoue, Sayori Tanaka, Satoka Doda, Shota Yamanaka, Hiroki Fukuchi, Yasuhiko Terada

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The centrosomal protein, CDK5RAP2, is a microcephaly protein that regulates centrosomal maturation by recruitment of a γ-tubulin ring complex (γ-TuRC) onto centrosomes. In this report, we identified a novel human centrosomal protein, Cep169, as a binding partner of CDK5RAP2, a member of microtubule plus-end-tracking proteins (+TIPs). Cep169 interacts directly with CDK5RAP2 through CM1, an evolutionarily conserved domain, and colocalizes at the pericentriolar matrix (PCM) around centrioles with CDK5RAP2. In addition, Cep169 interacts with EB1 through SxIP-motif responsible for EB1 binding, and colocalizes with CDK5RAP2 at the microtubule plus-end. EB1-binding-deficient Cep169 abolishes EB1 interaction and microtubule plus-end attachment, indicating Cep169 as a novel member of +TIPs. We further show that ectopic expression of either Cep169 or CDK5RAP2 induces microtubule bundling and acetylation in U2OS cells, and depletion of Cep169 induces microtubule depolymerization in HeLa cells, although Cep169 is not required for assembly of γ-tubulin onto centrosome by CDK5RAP2. These results show that Cep169 targets microtubule tips and regulates stability of microtubules with CDK5RAP2.

    Original languageEnglish
    Article numbere0140968
    JournalPLoS One
    Volume10
    Issue number10
    DOIs
    Publication statusPublished - 2015 Oct 20

    Fingerprint

    Microtubules
    microtubules
    Tubulin
    Proteins
    proteins
    centrosomes
    Centrosome
    Acetylation
    Depolymerization
    tubulin
    Centrioles
    Microcephaly
    centrioles
    depolymerization
    acetylation
    HeLa Cells
    cells

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Cep169, a novel microtubule plus-end-tracking centrosomal protein, binds to CDK5RAP2 and regulates microtubule stability. / Mori, Yusuke; Inoue, Yoko; Tanaka, Sayori; Doda, Satoka; Yamanaka, Shota; Fukuchi, Hiroki; Terada, Yasuhiko.

    In: PLoS One, Vol. 10, No. 10, e0140968, 20.10.2015.

    Research output: Contribution to journalArticle

    Mori, Yusuke ; Inoue, Yoko ; Tanaka, Sayori ; Doda, Satoka ; Yamanaka, Shota ; Fukuchi, Hiroki ; Terada, Yasuhiko. / Cep169, a novel microtubule plus-end-tracking centrosomal protein, binds to CDK5RAP2 and regulates microtubule stability. In: PLoS One. 2015 ; Vol. 10, No. 10.
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