Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease

Makoto Komiyama, Yota Takahashi, Naoya Takeda, Kazunari Matsumura, Teruyuki Kodama

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38 Citations (Scopus)

Abstract

Cerium(IV) ion is attached to the 5′ of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidyly1(3′-5)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalSupramolecular Chemistry
Volume4
Issue number1
DOIs
Publication statusPublished - 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)

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