Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease

Makoto Komiyama, Yota Takahashi, Naoya Takeda, Kazunari Matsumura, Teruyuki Kodama

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cerium(IV) ion is attached to the 5′ of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidyly1(3′-5)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalSupramolecular Chemistry
Volume4
Issue number1
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Cerium
DNA
Thymidine
Ions
Ribose
Hydrolysis
Ligands
Substrates

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease. / Komiyama, Makoto; Takahashi, Yota; Takeda, Naoya; Matsumura, Kazunari; Kodama, Teruyuki.

In: Supramolecular Chemistry, Vol. 4, No. 1, 1994, p. 31-34.

Research output: Contribution to journalArticle

Komiyama, Makoto ; Takahashi, Yota ; Takeda, Naoya ; Matsumura, Kazunari ; Kodama, Teruyuki. / Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease. In: Supramolecular Chemistry. 1994 ; Vol. 4, No. 1. pp. 31-34.
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