Change in PGC-1α expression in rat skeletal muscle after low-intensity prolonged swimming exercise

Eri Takai, Wataru Yamaguchi, Shin Terada, Mitsuru Higuchi, Izumi Tabata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

After 6-h low-intensity swimming exercise (LIE), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) in whole and nuclear fractions in rat skeletal muscle was higher than the control rats' muscles up to 18 h after LIE. However, no study has reported change in PGC-1α content after that. Therefore, we measured PGC-1α in whole and nuclear fractions in rat skeletal muscle up to 24 h after LIE. Furthermore, we evaluated change in the mRNA of δ-aminolevulinate synthase (ALAS), a mitochondrial protein, to clarify in which fraction of PGC-1α has a physiological role as a transcriptional coactivator for enhancing the mitochondrial oxidative enzymes after exercise. We measured PGC-1α protein content in whole and nuclear fractions in the epitrochlearis (EPI) muscle of male Sprague-Dawley rats (age: 6 w; body weight: 180-200 g) after LIE by Western-blot analysis. The ALAS mRNA content was quantified by RT-PCR. The PGC-1α contents in whole fractions in the rat EPI muscle were 73% and 75% higher than that of the control rats' muscle, 18 h and 24 h after LIE, respectively. The PGC-1α content in nuclear fractions in the muscle and ALAS mRNA was higher than that of the control rats' muscle by 58% and 25%, respectively, while they returned to the control level 24 h after LIE. The present investigation demonstrated that the time-course of PGC-1α content in nuclear fractions in the EPI muscle was the same as the ALAS mRNA, suggesting that PGC-1α in the nucleus may have a physiological function as a transcriptional coactivator for enhancing mitochondrial protein expression after exercise.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalJournal of Physiological Anthropology
Volume30
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

Muscle
Rats
Skeletal Muscle
Muscles
Rat control
Messenger RNA
Mitochondrial Proteins
Proteins
body weight
Peroxisome Proliferator-Activated Receptors
Swimming
Sprague Dawley Rats
Level control
Western Blotting
Body Weight
Polymerase Chain Reaction
Enzymes

Keywords

  • Low-intensity prolonged swimming exercise
  • PGC-1α
  • Rat
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Orthopedics and Sports Medicine
  • Physiology (medical)
  • Public Health, Environmental and Occupational Health
  • Human Factors and Ergonomics
  • Anthropology

Cite this

Change in PGC-1α expression in rat skeletal muscle after low-intensity prolonged swimming exercise. / Takai, Eri; Yamaguchi, Wataru; Terada, Shin; Higuchi, Mitsuru; Tabata, Izumi.

In: Journal of Physiological Anthropology, Vol. 30, No. 1, 2011, p. 23-27.

Research output: Contribution to journalArticle

Takai, Eri ; Yamaguchi, Wataru ; Terada, Shin ; Higuchi, Mitsuru ; Tabata, Izumi. / Change in PGC-1α expression in rat skeletal muscle after low-intensity prolonged swimming exercise. In: Journal of Physiological Anthropology. 2011 ; Vol. 30, No. 1. pp. 23-27.
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