Change in renal glomerular collagens and glomerular filtration barrier-related proteins in a dextran sulfate sodium-induced colitis mouse model

Chia Jung Chang, Pi Chao Wang, Tzou Chi Huang, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

Abstract

Renal disease is not rare among patients with inflammatory bowel disease (IBD) and is gaining interest as a target of research. However, related changes in glomerular structural have rarely been investigated. This study was aimed at clarifying the changes in collagens and glomerular filtration barrier (GFB)-related proteins of glomeruli in a dextran sulfate sodium (DSS)-induced colitis mouse model. Acute colitis was induced by administering 3.5% DSS in Slc:ICR strain mice for eight days. Histological changes to glomeruli were examined by periodic acid-Schiff (PAS) and Masson’s trichrome staining. Expressions of glomerular collagens and GFB-related proteins were analyzed by immunofluorescent staining and Western blot analysis. DSS-colitis mice showed an elevated disease activity index (DAI), colon shortening, massive cellular infiltration and colon damage, confirming that DSS-colitis mice can be used as an IBD animal model. DSS-colitis mice showed increased glycoprotein and collagen deposition in glomeruli. Interestingly, we observed significant changes in glomerular collagens, including a decrease in type IV collagen, and an increment in type I and type V collagens. Moreover, declined GFB-related proteins expressions were detected, including synaptopodin, podocalyxin, nephrin and VE-cadherin. These results suggest that renal disease in DSS-colitis mice might be associated with changes in glomerular collagens and GFB-related proteins. These findings are important for further elucidation of the clinical pathological mechanisms underlying IBD-associated renal disease.

Original languageEnglish
Article number1458
JournalInternational journal of molecular sciences
Volume20
Issue number6
DOIs
Publication statusPublished - 2019 Mar 2

Fingerprint

Glomerular Filtration Barrier
Dextran Sulfate
dextrans
Sodium sulfate
Dextran
Colitis
collagens
sodium sulfates
Collagen
mice
proteins
Proteins
Kidney
glomerulus
Inflammatory Bowel Diseases
staining
Colon
Collagen Type V
Staining and Labeling
Inbred ICR Mouse

Keywords

  • DSS-colitis
  • Glomerular filtration barrier (GFB)
  • Inflammatory bowel disease (IBD)
  • Type I collagen
  • Type IV collagen
  • Type V collagen

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Change in renal glomerular collagens and glomerular filtration barrier-related proteins in a dextran sulfate sodium-induced colitis mouse model. / Chang, Chia Jung; Wang, Pi Chao; Huang, Tzou Chi; Taniguchi, Akiyoshi.

In: International journal of molecular sciences, Vol. 20, No. 6, 1458, 02.03.2019.

Research output: Contribution to journalArticle

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