Change in the calcium-binding capacity of sea urchin egg homogenate caused by treatment with ATP and magnesium ions

M. Nakamura, I. Yasumasu

    Research output: Contribution to journalArticle

    Abstract

    The dissociation constant of the calcium and calcium-binding substance complex in sea urchin egg homogenate increased during incubation of the homogenate with CaCl2 at concentrations greater than 0.5 mM to the same value as that found in the fertilized egg homogenate. The dissociation constant of the calcium-binding substance in the unfertilized egg homogenate (pre-incubated with CaCl2), as well as the homogenate of the fertilized egg, was found to decrease to the value found in the unfertilized egg homogenate on treatment with 5 mM ATP and 5 mM MgCl2. It was found that a calcium-binding substance of the 'unfertilized egg type' was easily transformed to a 'fertilized egg type' by calcium ions and the fertilized egg type substance reversibly became an unfertilized egg type on treatment with ATP and Mg ions. This substance is therefore thought to play an important role in the regulation of the intracellular concentration of calcium.

    Original languageEnglish
    Pages (from-to)121-126
    Number of pages6
    JournalExperimental Cell Research
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - 1974

    Fingerprint

    Sea Urchins
    Magnesium
    Ovum
    Adenosine Triphosphate
    Zygote
    Ions
    Calcium
    Magnesium Chloride

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Change in the calcium-binding capacity of sea urchin egg homogenate caused by treatment with ATP and magnesium ions. / Nakamura, M.; Yasumasu, I.

    In: Experimental Cell Research, Vol. 88, No. 1, 1974, p. 121-126.

    Research output: Contribution to journalArticle

    @article{6a501ed46e3c48ed963091fb96ec88d8,
    title = "Change in the calcium-binding capacity of sea urchin egg homogenate caused by treatment with ATP and magnesium ions",
    abstract = "The dissociation constant of the calcium and calcium-binding substance complex in sea urchin egg homogenate increased during incubation of the homogenate with CaCl2 at concentrations greater than 0.5 mM to the same value as that found in the fertilized egg homogenate. The dissociation constant of the calcium-binding substance in the unfertilized egg homogenate (pre-incubated with CaCl2), as well as the homogenate of the fertilized egg, was found to decrease to the value found in the unfertilized egg homogenate on treatment with 5 mM ATP and 5 mM MgCl2. It was found that a calcium-binding substance of the 'unfertilized egg type' was easily transformed to a 'fertilized egg type' by calcium ions and the fertilized egg type substance reversibly became an unfertilized egg type on treatment with ATP and Mg ions. This substance is therefore thought to play an important role in the regulation of the intracellular concentration of calcium.",
    author = "M. Nakamura and I. Yasumasu",
    year = "1974",
    doi = "10.1016/0014-4827(74)90625-9",
    language = "English",
    volume = "88",
    pages = "121--126",
    journal = "Experimental Cell Research",
    issn = "0014-4827",
    publisher = "Academic Press Inc.",
    number = "1",

    }

    TY - JOUR

    T1 - Change in the calcium-binding capacity of sea urchin egg homogenate caused by treatment with ATP and magnesium ions

    AU - Nakamura, M.

    AU - Yasumasu, I.

    PY - 1974

    Y1 - 1974

    N2 - The dissociation constant of the calcium and calcium-binding substance complex in sea urchin egg homogenate increased during incubation of the homogenate with CaCl2 at concentrations greater than 0.5 mM to the same value as that found in the fertilized egg homogenate. The dissociation constant of the calcium-binding substance in the unfertilized egg homogenate (pre-incubated with CaCl2), as well as the homogenate of the fertilized egg, was found to decrease to the value found in the unfertilized egg homogenate on treatment with 5 mM ATP and 5 mM MgCl2. It was found that a calcium-binding substance of the 'unfertilized egg type' was easily transformed to a 'fertilized egg type' by calcium ions and the fertilized egg type substance reversibly became an unfertilized egg type on treatment with ATP and Mg ions. This substance is therefore thought to play an important role in the regulation of the intracellular concentration of calcium.

    AB - The dissociation constant of the calcium and calcium-binding substance complex in sea urchin egg homogenate increased during incubation of the homogenate with CaCl2 at concentrations greater than 0.5 mM to the same value as that found in the fertilized egg homogenate. The dissociation constant of the calcium-binding substance in the unfertilized egg homogenate (pre-incubated with CaCl2), as well as the homogenate of the fertilized egg, was found to decrease to the value found in the unfertilized egg homogenate on treatment with 5 mM ATP and 5 mM MgCl2. It was found that a calcium-binding substance of the 'unfertilized egg type' was easily transformed to a 'fertilized egg type' by calcium ions and the fertilized egg type substance reversibly became an unfertilized egg type on treatment with ATP and Mg ions. This substance is therefore thought to play an important role in the regulation of the intracellular concentration of calcium.

    UR - http://www.scopus.com/inward/record.url?scp=0016260977&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0016260977&partnerID=8YFLogxK

    U2 - 10.1016/0014-4827(74)90625-9

    DO - 10.1016/0014-4827(74)90625-9

    M3 - Article

    C2 - 4472785

    AN - SCOPUS:0016260977

    VL - 88

    SP - 121

    EP - 126

    JO - Experimental Cell Research

    JF - Experimental Cell Research

    SN - 0014-4827

    IS - 1

    ER -