Change of morphology and cytoskeletal protein gene expression during dibutyryl cAMP-induced differentiation in C6 glioma cells

Weiwei Hu, Takeshi Onuma, Naoko Birukawa, Masashi Abe, Etsuro Ito, Zhong Chen, Akihisa Urano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Elevation of the intracellular cAMP level induces morphological changes of astrocyte-like differentiation in C6 glioma cells. Such changes may be accompanied with expression of cytoskeletal protein genes. We therefore analyzed morphological changes after a treatment with dibutyryl cAMP (dbcAMP) and then assessed the expression of cytoskeletal protein genes by a quantitative real-time polymerase chain reaction. The cell number remained unaltered upon incubation with 1 mM dbcAMP in medium supplemented with 0.1% fetal bovine serum (FBS), whereas the number and lengths of processes increased, when compared with those of cells incubated in medium supplemented with 0.1% or 10% FBS only. The amounts of β-actin, γ-actin, and β-tubulin mRNAs in C6 cells, but not α-tubulin mRNA, increased during the early proliferation in DMEM containing 10% FBS. The expression of cytoskeletal protein genes decreased when incubated with 0.1% FBS or 1 mM dbcAMP in 0.1% FBS, compared with those of cells cultured in 10% FBS. These results indicated that, during the early proliferation in normal culture condition, the expression of cytoskeletal protein genes in C6 cells, except α-tubulin, increased, while in differentiating or differentiated C6 glioma cells, cAMP-induced morphological changes were not accompanied with elevation of gene expression for cytoskeletal proteins, such as actin and tubulin.

Original languageEnglish
Pages (from-to)519-528
Number of pages10
JournalCellular and Molecular Neurobiology
Volume28
Issue number4
DOIs
Publication statusPublished - 2008 Jun
Externally publishedYes

Fingerprint

Cytoskeletal Proteins
Gene expression
Glioma
Tubulin
Gene Expression
Genes
Actins
Serum
Messenger RNA
Polymerase chain reaction
Cell culture
Astrocytes
Real-Time Polymerase Chain Reaction
Cultured Cells
Cell Count

Keywords

  • Actin
  • C6 glioma cell
  • cAMP
  • Cytoskeleton
  • Differentiation
  • Gene expression
  • Morphology
  • Tubulin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Change of morphology and cytoskeletal protein gene expression during dibutyryl cAMP-induced differentiation in C6 glioma cells. / Hu, Weiwei; Onuma, Takeshi; Birukawa, Naoko; Abe, Masashi; Ito, Etsuro; Chen, Zhong; Urano, Akihisa.

In: Cellular and Molecular Neurobiology, Vol. 28, No. 4, 06.2008, p. 519-528.

Research output: Contribution to journalArticle

Hu, Weiwei ; Onuma, Takeshi ; Birukawa, Naoko ; Abe, Masashi ; Ito, Etsuro ; Chen, Zhong ; Urano, Akihisa. / Change of morphology and cytoskeletal protein gene expression during dibutyryl cAMP-induced differentiation in C6 glioma cells. In: Cellular and Molecular Neurobiology. 2008 ; Vol. 28, No. 4. pp. 519-528.
@article{fb5c830209544217bbd4d92e241efd72,
title = "Change of morphology and cytoskeletal protein gene expression during dibutyryl cAMP-induced differentiation in C6 glioma cells",
abstract = "Elevation of the intracellular cAMP level induces morphological changes of astrocyte-like differentiation in C6 glioma cells. Such changes may be accompanied with expression of cytoskeletal protein genes. We therefore analyzed morphological changes after a treatment with dibutyryl cAMP (dbcAMP) and then assessed the expression of cytoskeletal protein genes by a quantitative real-time polymerase chain reaction. The cell number remained unaltered upon incubation with 1 mM dbcAMP in medium supplemented with 0.1{\%} fetal bovine serum (FBS), whereas the number and lengths of processes increased, when compared with those of cells incubated in medium supplemented with 0.1{\%} or 10{\%} FBS only. The amounts of β-actin, γ-actin, and β-tubulin mRNAs in C6 cells, but not α-tubulin mRNA, increased during the early proliferation in DMEM containing 10{\%} FBS. The expression of cytoskeletal protein genes decreased when incubated with 0.1{\%} FBS or 1 mM dbcAMP in 0.1{\%} FBS, compared with those of cells cultured in 10{\%} FBS. These results indicated that, during the early proliferation in normal culture condition, the expression of cytoskeletal protein genes in C6 cells, except α-tubulin, increased, while in differentiating or differentiated C6 glioma cells, cAMP-induced morphological changes were not accompanied with elevation of gene expression for cytoskeletal proteins, such as actin and tubulin.",
keywords = "Actin, C6 glioma cell, cAMP, Cytoskeleton, Differentiation, Gene expression, Morphology, Tubulin",
author = "Weiwei Hu and Takeshi Onuma and Naoko Birukawa and Masashi Abe and Etsuro Ito and Zhong Chen and Akihisa Urano",
year = "2008",
month = "6",
doi = "10.1007/s10571-007-9229-y",
language = "English",
volume = "28",
pages = "519--528",
journal = "Cellular and Molecular Neurobiology",
issn = "0272-4340",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Change of morphology and cytoskeletal protein gene expression during dibutyryl cAMP-induced differentiation in C6 glioma cells

AU - Hu, Weiwei

AU - Onuma, Takeshi

AU - Birukawa, Naoko

AU - Abe, Masashi

AU - Ito, Etsuro

AU - Chen, Zhong

AU - Urano, Akihisa

PY - 2008/6

Y1 - 2008/6

N2 - Elevation of the intracellular cAMP level induces morphological changes of astrocyte-like differentiation in C6 glioma cells. Such changes may be accompanied with expression of cytoskeletal protein genes. We therefore analyzed morphological changes after a treatment with dibutyryl cAMP (dbcAMP) and then assessed the expression of cytoskeletal protein genes by a quantitative real-time polymerase chain reaction. The cell number remained unaltered upon incubation with 1 mM dbcAMP in medium supplemented with 0.1% fetal bovine serum (FBS), whereas the number and lengths of processes increased, when compared with those of cells incubated in medium supplemented with 0.1% or 10% FBS only. The amounts of β-actin, γ-actin, and β-tubulin mRNAs in C6 cells, but not α-tubulin mRNA, increased during the early proliferation in DMEM containing 10% FBS. The expression of cytoskeletal protein genes decreased when incubated with 0.1% FBS or 1 mM dbcAMP in 0.1% FBS, compared with those of cells cultured in 10% FBS. These results indicated that, during the early proliferation in normal culture condition, the expression of cytoskeletal protein genes in C6 cells, except α-tubulin, increased, while in differentiating or differentiated C6 glioma cells, cAMP-induced morphological changes were not accompanied with elevation of gene expression for cytoskeletal proteins, such as actin and tubulin.

AB - Elevation of the intracellular cAMP level induces morphological changes of astrocyte-like differentiation in C6 glioma cells. Such changes may be accompanied with expression of cytoskeletal protein genes. We therefore analyzed morphological changes after a treatment with dibutyryl cAMP (dbcAMP) and then assessed the expression of cytoskeletal protein genes by a quantitative real-time polymerase chain reaction. The cell number remained unaltered upon incubation with 1 mM dbcAMP in medium supplemented with 0.1% fetal bovine serum (FBS), whereas the number and lengths of processes increased, when compared with those of cells incubated in medium supplemented with 0.1% or 10% FBS only. The amounts of β-actin, γ-actin, and β-tubulin mRNAs in C6 cells, but not α-tubulin mRNA, increased during the early proliferation in DMEM containing 10% FBS. The expression of cytoskeletal protein genes decreased when incubated with 0.1% FBS or 1 mM dbcAMP in 0.1% FBS, compared with those of cells cultured in 10% FBS. These results indicated that, during the early proliferation in normal culture condition, the expression of cytoskeletal protein genes in C6 cells, except α-tubulin, increased, while in differentiating or differentiated C6 glioma cells, cAMP-induced morphological changes were not accompanied with elevation of gene expression for cytoskeletal proteins, such as actin and tubulin.

KW - Actin

KW - C6 glioma cell

KW - cAMP

KW - Cytoskeleton

KW - Differentiation

KW - Gene expression

KW - Morphology

KW - Tubulin

UR - http://www.scopus.com/inward/record.url?scp=43049141892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43049141892&partnerID=8YFLogxK

U2 - 10.1007/s10571-007-9229-y

DO - 10.1007/s10571-007-9229-y

M3 - Article

C2 - 18000753

AN - SCOPUS:43049141892

VL - 28

SP - 519

EP - 528

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

SN - 0272-4340

IS - 4

ER -