Changes in activities of glucose metabolising enzymes in germ cells during spermatogenesis in rats

M. Nakamura, A. Fujiwara, I. Yasumasu

Research output: Contribution to journalArticle

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Abstract

The rate of 14CO2 liberation from [14C-1]glucose was identical to that from [14C-6]glucose in spermatids, but more than the latter in spermatogonia. Rotenone (1 μM) completely inhibited 14CO2 release from [14C-1]glucose in spermatids, but decreased it only 30% in spermatogonia. The activity of glucose-6-phosphate dehydrogenase, but not 6-phosphogluconate dehydrogenase, was markedly lower in spermatocytes and spermatids than in spermatogonia. The activities of the glycolytic enzymes, glucosephosphate isomerase, fructose diphosphatase, glyceraldehyde-3-phosphate dehydrogenase and enolase, differed only slightly in spermatids and spermatogonia. It is concluded that the low glucose-6-phosphate dehydrogenase activity may contribute to the low activity of the pentose cycle in spermatocytes and spermatids.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalDevelopment Growth and Differentiation
Volume25
Issue number3
Publication statusPublished - 1983
Externally publishedYes

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Spermatids
Spermatogenesis
Spermatogonia
Germ Cells
Glucose
Enzymes
Spermatocytes
Glucosephosphate Dehydrogenase
Phosphogluconate Dehydrogenase
Glucose-6-Phosphate Isomerase
Activity Cycles
Pentoses
Rotenone
Glyceraldehyde-3-Phosphate Dehydrogenases
Phosphopyruvate Hydratase
Fructose

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy

Cite this

Changes in activities of glucose metabolising enzymes in germ cells during spermatogenesis in rats. / Nakamura, M.; Fujiwara, A.; Yasumasu, I.

In: Development Growth and Differentiation, Vol. 25, No. 3, 1983, p. 281-287.

Research output: Contribution to journalArticle

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