Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

Jonathan Peake, Gary Wilson, Matthew Hordern, Katsuhiko Suzuki, Kanemitsu Yamaya, Kazunori Nosaka, Laurel Mackinnon, Jeff S. Coombes

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Intense exercise stimulates the systemic release of a variety of factors that alter neutrophil surface receptor expression and functional activity. These alterations may influence resistance to infection after intense exercise. The aim of this study was to examine the influence of exercise intensity on neutrophil receptor expression, degranulation (measured by plasma and intracellular myeloperoxidase concentrations), and respiratory burst activity. Ten well-trained male runners ran on a treadmill for 60 min at 60% [moderate-intensity exercise (MI)] and 85% maximal oxygen consumption [high-intensity exercise (HI)]. Blood was drawn immediately before and after exercise and at I h postexercise. Immediately after HI, the expression of the neutrophil receptor CD16 was significantly below preexercise values (P < 0.01), whereas MI significantly reduced CD35 expression below preexercise values (P < 0.05). One hour after exercise at both intensities, there was a significant decline in CD11b expression (P < 0.05) and a further decrease in CD16 expression compared with preexercise values (P < 0.01). CD16 expression was lower 1 h after HI than 1 h after MI (P < 0.01). Immediately after HI, intracellular myeloperoxidase concentration was less than preexercise values (P < 0.01), whereas plasma myeloperoxidase concentration was greater (P < 0.01), indicating that HI stimulated neutrophil degranulation. Plasma myeloperoxidase concentration was higher immediately after HI than after MI (P < 0.01). Neutrophil respiratory burst activity increased after HI (P < 0.01). In summary, both MI and HI reduced neutrophil surface receptor expression. Although CD16 expression was reduced to a greater extent after HI, this reduction did not impair neutrophil degranulation and respiratory burst activity.

Original languageEnglish
Pages (from-to)612-618
Number of pages7
JournalJournal of Applied Physiology
Volume97
Issue number2
DOIs
Publication statusPublished - 2004 Aug

Fingerprint

Respiratory Burst
Neutrophils
Peroxidase
Oxygen Consumption
Infection

Keywords

  • CD16
  • Cytokines
  • Myeloperoxidase
  • Receptor shedding

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise. / Peake, Jonathan; Wilson, Gary; Hordern, Matthew; Suzuki, Katsuhiko; Yamaya, Kanemitsu; Nosaka, Kazunori; Mackinnon, Laurel; Coombes, Jeff S.

In: Journal of Applied Physiology, Vol. 97, No. 2, 08.2004, p. 612-618.

Research output: Contribution to journalArticle

Peake, Jonathan ; Wilson, Gary ; Hordern, Matthew ; Suzuki, Katsuhiko ; Yamaya, Kanemitsu ; Nosaka, Kazunori ; Mackinnon, Laurel ; Coombes, Jeff S. / Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise. In: Journal of Applied Physiology. 2004 ; Vol. 97, No. 2. pp. 612-618.
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