Changes in the bacterial community in the fermentation process of kôso, a Japanese sugar-vegetable fermented beverage

Tai Ying Chiou, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Tomoya Takahashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Kôso is a Japanese fermented beverage made with over 20 kinds of vegetables, mushrooms, and sugars. The changes in the bacterial population of kôso during fermentation at 25 ° C over a period of 10 days were studied using 454 pyrosequencing of the 16S rRNA gene. The analysis detected 224 operational taxonomic units (OTUs) clustered from 8 DNA samples collected on days 0, 3, 7, and 10 from two fermentation batches. Proteobacteria were the dominant phylum in the starting community, but were replaced by Firmicutes within three days. Seventy-eight genera were identified from the 224 OTUs, in which Bifidobacterium, Leuconostoc, Lactococcus, and Lactobacillus dominated, accounting for over 96% of the total bacterial population after three days' fermentation. UniFrac-Principal Coordinate Analysis of longitudinal fermented samples revealed dramatic changes in the bacterial community in kôso, resulting in significantly low diversity at the end of fermentation as compared with the complex starting community.

Original languageEnglish
Pages (from-to)403-410
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume81
Issue number2
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Beverages
Vegetables
Sugars
Fermentation
Lactococcus
Leuconostoc
Proteobacteria
Bifidobacterium
Agaricales
Lactobacillus
rRNA Genes
Population
Genes
DNA

Keywords

  • Bacterial community
  • Kôso
  • Lactic acid bacteria
  • Spontaneous fermentation
  • Sugar-vegetable fermented beverage

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Changes in the bacterial community in the fermentation process of kôso, a Japanese sugar-vegetable fermented beverage. / Chiou, Tai Ying; Suda, Wataru; Oshima, Kenshiro; Hattori, Masahira; Takahashi, Tomoya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 81, No. 2, 2017, p. 403-410.

Research output: Contribution to journalArticle

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