Changes in Two Kinds of 20α-Hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat

Ken Noda, Kunio Shiota, Hwan Hoo Seong, Michio Takahashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have found previously that there are 2 kinds of 20α-hydroxysteroid dehydrogenase (20α-HSD) (EC 1.1.1.149) molecule (HSD1 and HSD2) in the mature rat ovary. In the present study, each enzyme activity was measured during pseudopregnancy (PSP) in the rat. Ovarian cytosol was collected in early (day 5), mid (day 9) or late (day 15) PSP. HSD1 activity was low in early and mid PSP and increased markedly at the end of PSP, while HSD2 activity was lower than HSD1 activity in early PSP and decreased as PSP advanced. These results suggest that the regulatory mechanism of the enzyme activity varies between HSD1 and HSD2, and that HSD1 mainly contributes to the increase in 20α-dihydroprogesterone secretion at the end of PSP. Interestingly, when ovaries at early and mid-term PSP were used, the total 20α-HSD activity in the cytosol fraction was increased markedly (163-296%) by passage through a DEAE column. This phenomenon was not observed with ovarian cytosol obtained at late PSP. Thus, we conclude that the ovary contains 2 different kinds of 20α-HSD molecule whose activities seem to be regulated separatedly, and that the HSD1 molecule is responsible for termination of the luteal phase.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalJournal of Reproduction and Development
Volume38
Issue number1
DOIs
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

pseudopregnancy
hydroxysteroid dehydrogenases
corpus luteum
rats
cytosol
enzyme activity
secretion

Keywords

  • 20α-dihydroprogesterone
  • 20α-hydroxysteroid dehydrogenase
  • HSD1
  • HSD2
  • pseudopregnancy

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Changes in Two Kinds of 20α-Hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat. / Noda, Ken; Shiota, Kunio; Seong, Hwan Hoo; Takahashi, Michio.

In: Journal of Reproduction and Development, Vol. 38, No. 1, 1992, p. 49-53.

Research output: Contribution to journalArticle

Noda, Ken ; Shiota, Kunio ; Seong, Hwan Hoo ; Takahashi, Michio. / Changes in Two Kinds of 20α-Hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat. In: Journal of Reproduction and Development. 1992 ; Vol. 38, No. 1. pp. 49-53.
@article{0ae3fd03515b4d32816f517e7657c18d,
title = "Changes in Two Kinds of 20α-Hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat",
abstract = "We have found previously that there are 2 kinds of 20α-hydroxysteroid dehydrogenase (20α-HSD) (EC 1.1.1.149) molecule (HSD1 and HSD2) in the mature rat ovary. In the present study, each enzyme activity was measured during pseudopregnancy (PSP) in the rat. Ovarian cytosol was collected in early (day 5), mid (day 9) or late (day 15) PSP. HSD1 activity was low in early and mid PSP and increased markedly at the end of PSP, while HSD2 activity was lower than HSD1 activity in early PSP and decreased as PSP advanced. These results suggest that the regulatory mechanism of the enzyme activity varies between HSD1 and HSD2, and that HSD1 mainly contributes to the increase in 20α-dihydroprogesterone secretion at the end of PSP. Interestingly, when ovaries at early and mid-term PSP were used, the total 20α-HSD activity in the cytosol fraction was increased markedly (163-296{\%}) by passage through a DEAE column. This phenomenon was not observed with ovarian cytosol obtained at late PSP. Thus, we conclude that the ovary contains 2 different kinds of 20α-HSD molecule whose activities seem to be regulated separatedly, and that the HSD1 molecule is responsible for termination of the luteal phase.",
keywords = "20α-dihydroprogesterone, 20α-hydroxysteroid dehydrogenase, HSD1, HSD2, pseudopregnancy",
author = "Ken Noda and Kunio Shiota and Seong, {Hwan Hoo} and Michio Takahashi",
year = "1992",
doi = "10.1262/jrd.38.49",
language = "English",
volume = "38",
pages = "49--53",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "1",

}

TY - JOUR

T1 - Changes in Two Kinds of 20α-Hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat

AU - Noda, Ken

AU - Shiota, Kunio

AU - Seong, Hwan Hoo

AU - Takahashi, Michio

PY - 1992

Y1 - 1992

N2 - We have found previously that there are 2 kinds of 20α-hydroxysteroid dehydrogenase (20α-HSD) (EC 1.1.1.149) molecule (HSD1 and HSD2) in the mature rat ovary. In the present study, each enzyme activity was measured during pseudopregnancy (PSP) in the rat. Ovarian cytosol was collected in early (day 5), mid (day 9) or late (day 15) PSP. HSD1 activity was low in early and mid PSP and increased markedly at the end of PSP, while HSD2 activity was lower than HSD1 activity in early PSP and decreased as PSP advanced. These results suggest that the regulatory mechanism of the enzyme activity varies between HSD1 and HSD2, and that HSD1 mainly contributes to the increase in 20α-dihydroprogesterone secretion at the end of PSP. Interestingly, when ovaries at early and mid-term PSP were used, the total 20α-HSD activity in the cytosol fraction was increased markedly (163-296%) by passage through a DEAE column. This phenomenon was not observed with ovarian cytosol obtained at late PSP. Thus, we conclude that the ovary contains 2 different kinds of 20α-HSD molecule whose activities seem to be regulated separatedly, and that the HSD1 molecule is responsible for termination of the luteal phase.

AB - We have found previously that there are 2 kinds of 20α-hydroxysteroid dehydrogenase (20α-HSD) (EC 1.1.1.149) molecule (HSD1 and HSD2) in the mature rat ovary. In the present study, each enzyme activity was measured during pseudopregnancy (PSP) in the rat. Ovarian cytosol was collected in early (day 5), mid (day 9) or late (day 15) PSP. HSD1 activity was low in early and mid PSP and increased markedly at the end of PSP, while HSD2 activity was lower than HSD1 activity in early PSP and decreased as PSP advanced. These results suggest that the regulatory mechanism of the enzyme activity varies between HSD1 and HSD2, and that HSD1 mainly contributes to the increase in 20α-dihydroprogesterone secretion at the end of PSP. Interestingly, when ovaries at early and mid-term PSP were used, the total 20α-HSD activity in the cytosol fraction was increased markedly (163-296%) by passage through a DEAE column. This phenomenon was not observed with ovarian cytosol obtained at late PSP. Thus, we conclude that the ovary contains 2 different kinds of 20α-HSD molecule whose activities seem to be regulated separatedly, and that the HSD1 molecule is responsible for termination of the luteal phase.

KW - 20α-dihydroprogesterone

KW - 20α-hydroxysteroid dehydrogenase

KW - HSD1

KW - HSD2

KW - pseudopregnancy

UR - http://www.scopus.com/inward/record.url?scp=85008296425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008296425&partnerID=8YFLogxK

U2 - 10.1262/jrd.38.49

DO - 10.1262/jrd.38.49

M3 - Article

AN - SCOPUS:85008296425

VL - 38

SP - 49

EP - 53

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 1

ER -