Chaotropic etching for fabricating microwells for cell culture

Nobuhiro Tamai, Hiroyuki Moriguchi, Yuzo Takayama, Yasuhiko Jimbo, Ikuro Suzuki, Kenji Yasuda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Formation of simple neuronal networks in vitro is one of the promising methods to study biological information processing. Agarose microchambers have several advantages to form and maintain simple network structures. Here, in this work, a novel method for fabricating microwells in an agarose-layer is reported. Chaotropic effects of sodium iodide (NaI) is applied for etching agarose films. A conventional glass micropipette filled with NaI solution was aligned and a small amount of NaI was ejected to surface of the film. The agarose was denatured by the soaked NaI. The denatured agarose was washed out by distilled water. The size of the well was determined by the quantity of ejected NaI solution and its diffusion time. Conditions for fabricating wells of 100 to 600 μm diameters were established. Multiple wells up to 100 were formed on a single surface sequentially by programmed movement of the microscope stage. Rat hippocampal neurons were successfully cultured in the wells. Combining this method with microelectrode-array substrates will enable us of recording neuronal activity from simple neuronal networks as well as co-culture systems of heterogeneous tissues.

Original languageEnglish
JournalIEEJ Transactions on Electronics, Information and Systems
Volume127
Issue number10
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Cell culture
Etching
Microelectrodes
Neurons
Rats
Microscopes
Sodium
Tissue
Glass
Substrates
Water

Keywords

  • Cell culture
  • Chaotropic effect
  • Directional growth
  • Micro-fabrication
  • Neuron

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Chaotropic etching for fabricating microwells for cell culture. / Tamai, Nobuhiro; Moriguchi, Hiroyuki; Takayama, Yuzo; Jimbo, Yasuhiko; Suzuki, Ikuro; Yasuda, Kenji.

In: IEEJ Transactions on Electronics, Information and Systems, Vol. 127, No. 10, 2007.

Research output: Contribution to journalArticle

Tamai, Nobuhiro ; Moriguchi, Hiroyuki ; Takayama, Yuzo ; Jimbo, Yasuhiko ; Suzuki, Ikuro ; Yasuda, Kenji. / Chaotropic etching for fabricating microwells for cell culture. In: IEEJ Transactions on Electronics, Information and Systems. 2007 ; Vol. 127, No. 10.
@article{4f50fdde2bb7413688535ea4c6a7b690,
title = "Chaotropic etching for fabricating microwells for cell culture",
abstract = "Formation of simple neuronal networks in vitro is one of the promising methods to study biological information processing. Agarose microchambers have several advantages to form and maintain simple network structures. Here, in this work, a novel method for fabricating microwells in an agarose-layer is reported. Chaotropic effects of sodium iodide (NaI) is applied for etching agarose films. A conventional glass micropipette filled with NaI solution was aligned and a small amount of NaI was ejected to surface of the film. The agarose was denatured by the soaked NaI. The denatured agarose was washed out by distilled water. The size of the well was determined by the quantity of ejected NaI solution and its diffusion time. Conditions for fabricating wells of 100 to 600 μm diameters were established. Multiple wells up to 100 were formed on a single surface sequentially by programmed movement of the microscope stage. Rat hippocampal neurons were successfully cultured in the wells. Combining this method with microelectrode-array substrates will enable us of recording neuronal activity from simple neuronal networks as well as co-culture systems of heterogeneous tissues.",
keywords = "Cell culture, Chaotropic effect, Directional growth, Micro-fabrication, Neuron",
author = "Nobuhiro Tamai and Hiroyuki Moriguchi and Yuzo Takayama and Yasuhiko Jimbo and Ikuro Suzuki and Kenji Yasuda",
year = "2007",
doi = "10.1541/ieejeiss.127.1568",
language = "English",
volume = "127",
journal = "IEEJ Transactions on Electronics, Information and Systems",
issn = "0385-4221",
publisher = "The Institute of Electrical Engineers of Japan",
number = "10",

}

TY - JOUR

T1 - Chaotropic etching for fabricating microwells for cell culture

AU - Tamai, Nobuhiro

AU - Moriguchi, Hiroyuki

AU - Takayama, Yuzo

AU - Jimbo, Yasuhiko

AU - Suzuki, Ikuro

AU - Yasuda, Kenji

PY - 2007

Y1 - 2007

N2 - Formation of simple neuronal networks in vitro is one of the promising methods to study biological information processing. Agarose microchambers have several advantages to form and maintain simple network structures. Here, in this work, a novel method for fabricating microwells in an agarose-layer is reported. Chaotropic effects of sodium iodide (NaI) is applied for etching agarose films. A conventional glass micropipette filled with NaI solution was aligned and a small amount of NaI was ejected to surface of the film. The agarose was denatured by the soaked NaI. The denatured agarose was washed out by distilled water. The size of the well was determined by the quantity of ejected NaI solution and its diffusion time. Conditions for fabricating wells of 100 to 600 μm diameters were established. Multiple wells up to 100 were formed on a single surface sequentially by programmed movement of the microscope stage. Rat hippocampal neurons were successfully cultured in the wells. Combining this method with microelectrode-array substrates will enable us of recording neuronal activity from simple neuronal networks as well as co-culture systems of heterogeneous tissues.

AB - Formation of simple neuronal networks in vitro is one of the promising methods to study biological information processing. Agarose microchambers have several advantages to form and maintain simple network structures. Here, in this work, a novel method for fabricating microwells in an agarose-layer is reported. Chaotropic effects of sodium iodide (NaI) is applied for etching agarose films. A conventional glass micropipette filled with NaI solution was aligned and a small amount of NaI was ejected to surface of the film. The agarose was denatured by the soaked NaI. The denatured agarose was washed out by distilled water. The size of the well was determined by the quantity of ejected NaI solution and its diffusion time. Conditions for fabricating wells of 100 to 600 μm diameters were established. Multiple wells up to 100 were formed on a single surface sequentially by programmed movement of the microscope stage. Rat hippocampal neurons were successfully cultured in the wells. Combining this method with microelectrode-array substrates will enable us of recording neuronal activity from simple neuronal networks as well as co-culture systems of heterogeneous tissues.

KW - Cell culture

KW - Chaotropic effect

KW - Directional growth

KW - Micro-fabrication

KW - Neuron

UR - http://www.scopus.com/inward/record.url?scp=72149115661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72149115661&partnerID=8YFLogxK

U2 - 10.1541/ieejeiss.127.1568

DO - 10.1541/ieejeiss.127.1568

M3 - Article

AN - SCOPUS:72149115661

VL - 127

JO - IEEJ Transactions on Electronics, Information and Systems

JF - IEEJ Transactions on Electronics, Information and Systems

SN - 0385-4221

IS - 10

ER -