Chaperone properties of mammalian mitochondrial translation elongation factor Tu

Hiroaki Suzuki, Takuya Ueda, Hideki Taguchi, Nono Takeuchi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The main function of the prokaryotic translation elongation factor Tu (EF-Tu) and its eukaryotic counterpart eEF1A is to deliver aminoacyl-tRNA to the A-site on the ribosome. In addition to this primary function, it has been reported that EF-Tu from various sources has chaperone activity. At present, little information is available about the chaperone activity of mitochondrial EF-Tu. In the present study, we have examined the chaperone function of mammalian mitochondrial EF-Tu (EF-Tumt). We demonstrate that recombinant EF-Tumt prevents thermal aggregation of proteins and enhances protein refolding in vitro and that this EF-Tumt chaperone activity proceeds in a GTP-independent manner. We also demonstrate that, under heat stress, the newly synthesized peptides from the mitochondrial ribosome specifically co-immunoprecipitate with EF-Tumt and are destabilized in EF-Tumt-overexpressing cells. We show that most of the EF-Tumt localizes on the mitochondrial inner membrane where most mitochondrial ribosomes are found. We discuss the possible role of EF-Tumt chaperone activity in protein quality control in mitochondria, with regard to the recently reported in vivo chaperone function of eEF1A.

Original languageEnglish
Pages (from-to)4076-4084
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number6
DOIs
Publication statusPublished - 2007 Jan 9
Externally publishedYes

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Peptide Elongation Factor Tu
Hot Temperature
Protein Refolding
Mitochondrial Membranes
Transfer RNA
Guanosine Triphosphate
Ribosomes
Quality Control
Proteins
Mitochondria
Quality control
Peptides
Agglomeration
Membranes
Mitochondrial Ribosomes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chaperone properties of mammalian mitochondrial translation elongation factor Tu. / Suzuki, Hiroaki; Ueda, Takuya; Taguchi, Hideki; Takeuchi, Nono.

In: Journal of Biological Chemistry, Vol. 282, No. 6, 09.01.2007, p. 4076-4084.

Research output: Contribution to journalArticle

Suzuki, Hiroaki ; Ueda, Takuya ; Taguchi, Hideki ; Takeuchi, Nono. / Chaperone properties of mammalian mitochondrial translation elongation factor Tu. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 6. pp. 4076-4084.
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