Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier

Hiromi Sakai, Keitaro Sou, Eishun Tsuchida

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Hemoglobin-vesicles (HbV) or liposome-encapsulated hemoglobin (LEH) are artificial oxygen carriers that mimic the cellular structure of RBCs. In contrast to other liposomal products containing antifungal or anticancer drugs, one injection of HbV in place of a blood transfusion is estimated as equivalent to a massive dose, such as several hundred milliliters or a few liters of normal blood contents. The fluid must therefore contain a sufficient amount of Hb, the binding site of oxygen, to carry oxygen like blood. Encapsulation of Hb can shield various toxic effects of molecular Hbs. On the other hand, the liposomal structure, surface property, and the balance between the stability for storage and blood circulation and instability for the prompt degradation in the reticuloendothelial system must be considered to establish an optimal transfusion alternative.

Original languageEnglish
Pages (from-to)363-384
Number of pages22
JournalMethods in Enzymology
Volume465
Issue numberC
DOIs
Publication statusPublished - 2009

Fingerprint

Hemoglobins
Blood
Oxygen
Blood Substitutes
Mononuclear Phagocyte System
Surface Properties
Blood Circulation
Poisons
Hemodynamics
Cellular Structures
Encapsulation
Liposomes
Blood Transfusion
Surface properties
Binding Sites
Degradation
Injections
Fluids
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier. / Sakai, Hiromi; Sou, Keitaro; Tsuchida, Eishun.

In: Methods in Enzymology, Vol. 465, No. C, 2009, p. 363-384.

Research output: Contribution to journalArticle

Sakai, Hiromi ; Sou, Keitaro ; Tsuchida, Eishun. / Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier. In: Methods in Enzymology. 2009 ; Vol. 465, No. C. pp. 363-384.
@article{de41d2311b9a418b8aaabecf19a0e6a9,
title = "Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier",
abstract = "Hemoglobin-vesicles (HbV) or liposome-encapsulated hemoglobin (LEH) are artificial oxygen carriers that mimic the cellular structure of RBCs. In contrast to other liposomal products containing antifungal or anticancer drugs, one injection of HbV in place of a blood transfusion is estimated as equivalent to a massive dose, such as several hundred milliliters or a few liters of normal blood contents. The fluid must therefore contain a sufficient amount of Hb, the binding site of oxygen, to carry oxygen like blood. Encapsulation of Hb can shield various toxic effects of molecular Hbs. On the other hand, the liposomal structure, surface property, and the balance between the stability for storage and blood circulation and instability for the prompt degradation in the reticuloendothelial system must be considered to establish an optimal transfusion alternative.",
author = "Hiromi Sakai and Keitaro Sou and Eishun Tsuchida",
year = "2009",
doi = "10.1016/S0076-6879(09)65019-9",
language = "English",
volume = "465",
pages = "363--384",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",

}

TY - JOUR

T1 - Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier

AU - Sakai, Hiromi

AU - Sou, Keitaro

AU - Tsuchida, Eishun

PY - 2009

Y1 - 2009

N2 - Hemoglobin-vesicles (HbV) or liposome-encapsulated hemoglobin (LEH) are artificial oxygen carriers that mimic the cellular structure of RBCs. In contrast to other liposomal products containing antifungal or anticancer drugs, one injection of HbV in place of a blood transfusion is estimated as equivalent to a massive dose, such as several hundred milliliters or a few liters of normal blood contents. The fluid must therefore contain a sufficient amount of Hb, the binding site of oxygen, to carry oxygen like blood. Encapsulation of Hb can shield various toxic effects of molecular Hbs. On the other hand, the liposomal structure, surface property, and the balance between the stability for storage and blood circulation and instability for the prompt degradation in the reticuloendothelial system must be considered to establish an optimal transfusion alternative.

AB - Hemoglobin-vesicles (HbV) or liposome-encapsulated hemoglobin (LEH) are artificial oxygen carriers that mimic the cellular structure of RBCs. In contrast to other liposomal products containing antifungal or anticancer drugs, one injection of HbV in place of a blood transfusion is estimated as equivalent to a massive dose, such as several hundred milliliters or a few liters of normal blood contents. The fluid must therefore contain a sufficient amount of Hb, the binding site of oxygen, to carry oxygen like blood. Encapsulation of Hb can shield various toxic effects of molecular Hbs. On the other hand, the liposomal structure, surface property, and the balance between the stability for storage and blood circulation and instability for the prompt degradation in the reticuloendothelial system must be considered to establish an optimal transfusion alternative.

UR - http://www.scopus.com/inward/record.url?scp=71849095830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71849095830&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(09)65019-9

DO - 10.1016/S0076-6879(09)65019-9

M3 - Article

C2 - 19913177

AN - SCOPUS:71849095830

VL - 465

SP - 363

EP - 384

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

IS - C

ER -